Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | about 100% activation at 10 mM | Escherichia coli | |
K+ | activates NADH production | Escherichia coli | |
NH4+ | activates NADH production | Escherichia coli |
Application | Comment | Organism |
---|---|---|
molecular biology | enzymatic redox cofactor regeneration in organic media: functionalization and application of recombinant glycerol dehydrogenase and soluble transhydrogenase in reverse micelles, overview | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression as His-tag fusion protein in Escherichia coli BL21 (DE3) pLysS | Escherichia coli |
gene gld, expression of His-tagged enzyme in strain BL21(DE3) | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NH4+ | 80% activation at 10 mM | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Escherichia coli | - |
D-glyceraldehyde + NADH + H+ | - |
r | |
glycerol + NAD+ | Escherichia coli JM109 | - |
D-glyceraldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
gene gld | - |
Escherichia coli JM109 | - |
gene gld | - |
Purification (Comment) | Organism |
---|---|
purified by Ni-affinity chromatography | Escherichia coli |
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay development for NADH production by recombinant enzyme in reverse micelles, micelle size optimization, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate | Escherichia coli | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | the reaction is performed in reverse micelles harboring glycerol and NAD+ in a solution of isooctane containing 250 mM diocytlsulfosuccinate | Escherichia coli JM109 | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Escherichia coli | D-glyceraldehyde + NADH + H+ | - |
r | |
glycerol + NAD+ | - |
Escherichia coli JM109 | D-glyceraldehyde + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GLD | - |
Escherichia coli |
glycerol dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, in reverse micelles | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
in an aqueous buffer system | Escherichia coli |
10.5 | - |
in reverse micelles | Escherichia coli |
10.5 | 11 | NADH production | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ and NADPH | Escherichia coli | |
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli |