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Literature summary for 1.1.1.6 extracted from

  • Zhang, J.; Cui, Z.; Chang, H.; Fan, X.; Zhao, Q.; Wei, W.
    Conversion of glycerol to 1,3-dihydroxyacetone by glycerol dehydrogenase co-expressed with an NADH oxidase for cofactor regeneration (2016), Biotechnol. Lett., 38, 1559-1564.
    View publication on PubMed

Application

Application Comment Organism
synthesis efficiency of a cofactor regeneration enzyme co-expressed with a glycerol dehydrogenase for the production of 1,3-dihydroxyacetone. In vitro biotransformation of glycerol is achieved with the cell-free extracts containing recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, or NADH oxidase LpNox1 from Lactobacillus pentosus, giving1,3-dihydroxyacetone (DHA), no expensive consumption of NAD+ for the production of DHA, overview. DHA is a valuable chemical with a wide range of applications in the cosmetics Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of enzymes recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, and NADH oxidase LpNox1 from Lactobacillus pentosus in Escherichia coli strain BL21(DE3). The activities of LDH, LpNox1 and GlyDH in the cell-free extract are 98.8, 27.8 and 39.5 U/ml, respectively, overview Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information efficiency of a cofactor regeneration enzyme co-expressed with a glycerol dehydrogenase for the production of 1,3-dihydroxyacetone. In vitro biotransformation of glycerol is achieved with the cell-free extracts containing recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, or NADH oxidase LpNox1 from Lactobacillus pentosus, giving1,3-dihydroxyacetone, coexpression of all enzymes in Escherichia coli strain BL21(DE3) Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, recombinant enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Escherichia coli
-
glycerone + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + NAD+
-
Escherichia coli glycerone + NADH + H+
-
r
additional information efficiency of a cofactor regeneration enzyme co-expressed with a glycerol dehydrogenase for the production of 1,3-dihydroxyacetone. In vitro biotransformation of glycerol is achieved with the cell-free extracts containing recombinant glycerol dehydrogenase from Escherichia coli, lactate dehydrogenase form Bacillus subtilis, or NADH oxidase LpNox1 from Lactobacillus pentosus, giving1,3-dihydroxyacetone, overview Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 40000, recombinant enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
GlyDH
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at, purified enzyme Escherichia coli
30
-
assay at, recombinant whole cell extract Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli