BRENDA - Enzyme Database
show all sequences of 1.1.1.6

Bioinspired immobilization of glycerol dehydrogenase by metal ion-chelated polyethyleneimines as artificial polypeptides

Zhang, Y.; Ren, H.; Wang, Y.; Chen, K.; Fang, B.; Wang, S.; Sci. Rep. 6, 24163 (2016)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
polyethyleneimines
PEI, activating at 0.5 mM, inhibitory at 1 mM
Klebsiella pneumoniae
Cloned(Commentary)
Commentary
Organism
recombinent expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pET-32a
Klebsiella pneumoniae
Engineering
Amino acid exchange
Commentary
Organism
additional information
bioinspired immobilization of glycerol dehydrogenase by metal ion-chelated polyethyleneimines (PEI) as artificial polypeptides. Nanoparticles with diameters from 250–650 nm are prepared that exhibit a 1.4fold enhancement catalytic efficiency. The oligomeric GDH assemblies are coated and stabilized by the excessive manganese-chelated PEIs, which further prevents the disassociation of the GDH subunits, metal-mediated oligomeric assemblies of the enzyme. Half-life of immobilized GDH is enhanced by 5.6folds in aqueous phase at 85°C. Formation of multi-level interactions in the PEI-metal-GDH complex, mechanism, overview. A potential technique for multimeric enzyme immobilization with the advantages of low cost, easy operation, high activity reservation, and high stability. The activity of PEI-Mn2+-GDH gradually decreases over 5 cycles. PEI-Mn2+-GDH retains 71% and 53% of its initial activity after cycling through 3 and 5 successive reactions, respectively. The decrease in the activity of the recycled catalyst may be due to the leakage of GDH
Klebsiella pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
Cu2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
Mg2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
Ni2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
polyethyleneimines
activating at 0.5 mM, inhibitory at 1 mM
Klebsiella pneumoniae
Zn2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
Klebsiella pneumoniae
21.6
-
glycerol
pH 10.0, 30°C, free enzyme
Klebsiella pneumoniae
26
-
glycerol
pH 10.0, 30°C, immobilized enzyme
Klebsiella pneumoniae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
activates, polyethyleneimines-immobilized enzyme PEI-Mn2+-GDH exhibits a 2.9fold increase in activity compared with free GDH
Klebsiella pneumoniae
additional information
the natural GDH-bound Zn2+ are substituted with several divalent metal ions and the enzyme activity is significantly altered. Cu2+, Ni2+, Zn2+, Mn2+, Mg2+ and Ca2+ are used to investigate the effects of metal ions on GDH activity. Only Mn2+ improves the GDH activity by 1.1fold, whereas the other five metal ions inhibit the enzyme to varying degrees at high concentrations
Klebsiella pneumoniae
Zn2+
required
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NAD+
Klebsiella pneumoniae
-
glycerone + NADH + H+
-
-
r
glycerol + NAD+
Klebsiella pneumoniae DSM 2026
-
glycerone + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
B2ZPN8
-
-
Klebsiella pneumoniae DSM 2026
B2ZPN8
-
-
Purification (Commentary)
Commentary
Organism
recombinent His-tagged enzyme from Escherichia coli strain BL21(DE3) pET-32a by nickel affinity chromatography, desalting gel filtration, and ultrafiltration
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerol + NAD+
-
741417
Klebsiella pneumoniae
glycerone + NADH + H+
-
-
-
r
glycerol + NAD+
-
741417
Klebsiella pneumoniae DSM 2026
glycerone + NADH + H+
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Klebsiella pneumoniae
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
additional information
-
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
Klebsiella pneumoniae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
metal ion-chelated polyethyleneimine-immobilized enzyme PEI-Mn2+-GDH exhibits greater heat resistance than free enzyme GDH. After incubation for 90 min at 70°C, PEI-Mn2+-GDH still presents nearly 50% of its initial activity, while free GDH is almost totally inactivated
Klebsiella pneumoniae
85
-
metal ion-chelated polyethyleneimine-immobilized enzyme PEI-Mn2+-GDH exhibits a half-life that is enhanced by 5.6folds in aqueous phase at 85°C
Klebsiella pneumoniae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.06
-
glycerol
pH 10.0, 30°C, free enzyme
Klebsiella pneumoniae
3.36
-
glycerol
pH 10.0, 30°C, immobilized enzyme
Klebsiella pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
Klebsiella pneumoniae
10
11
the immobilized GDH changes the optimal pH from pH 11 to 10 and exhibits high activity in a relatively wide range of pH from pH 7 to 12, while free GDH only achieves high activity at pH 11
Klebsiella pneumoniae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
immobilization of the enzyme by metal ion-chelated polyethyleneimines greatly broadens the pH adaptability of GDH from strong alkaline into alkalescence
Klebsiella pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Klebsiella pneumoniae
NADH
-
Klebsiella pneumoniae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
polyethyleneimines
PEI, activating at 0.5 mM, inhibitory at 1 mM
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinent expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pET-32a
Klebsiella pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Klebsiella pneumoniae
NADH
-
Klebsiella pneumoniae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
bioinspired immobilization of glycerol dehydrogenase by metal ion-chelated polyethyleneimines (PEI) as artificial polypeptides. Nanoparticles with diameters from 250–650 nm are prepared that exhibit a 1.4fold enhancement catalytic efficiency. The oligomeric GDH assemblies are coated and stabilized by the excessive manganese-chelated PEIs, which further prevents the disassociation of the GDH subunits, metal-mediated oligomeric assemblies of the enzyme. Half-life of immobilized GDH is enhanced by 5.6folds in aqueous phase at 85°C. Formation of multi-level interactions in the PEI-metal-GDH complex, mechanism, overview. A potential technique for multimeric enzyme immobilization with the advantages of low cost, easy operation, high activity reservation, and high stability. The activity of PEI-Mn2+-GDH gradually decreases over 5 cycles. PEI-Mn2+-GDH retains 71% and 53% of its initial activity after cycling through 3 and 5 successive reactions, respectively. The decrease in the activity of the recycled catalyst may be due to the leakage of GDH
Klebsiella pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
Cu2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
Mg2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
Ni2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
polyethyleneimines
activating at 0.5 mM, inhibitory at 1 mM
Klebsiella pneumoniae
Zn2+
inhibits the enzyme at high concentrations
Klebsiella pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
Klebsiella pneumoniae
21.6
-
glycerol
pH 10.0, 30°C, free enzyme
Klebsiella pneumoniae
26
-
glycerol
pH 10.0, 30°C, immobilized enzyme
Klebsiella pneumoniae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
activates, polyethyleneimines-immobilized enzyme PEI-Mn2+-GDH exhibits a 2.9fold increase in activity compared with free GDH
Klebsiella pneumoniae
additional information
the natural GDH-bound Zn2+ are substituted with several divalent metal ions and the enzyme activity is significantly altered. Cu2+, Ni2+, Zn2+, Mn2+, Mg2+ and Ca2+ are used to investigate the effects of metal ions on GDH activity. Only Mn2+ improves the GDH activity by 1.1fold, whereas the other five metal ions inhibit the enzyme to varying degrees at high concentrations
Klebsiella pneumoniae
Zn2+
required
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerol + NAD+
Klebsiella pneumoniae
-
glycerone + NADH + H+
-
-
r
glycerol + NAD+
Klebsiella pneumoniae DSM 2026
-
glycerone + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinent His-tagged enzyme from Escherichia coli strain BL21(DE3) pET-32a by nickel affinity chromatography, desalting gel filtration, and ultrafiltration
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerol + NAD+
-
741417
Klebsiella pneumoniae
glycerone + NADH + H+
-
-
-
r
glycerol + NAD+
-
741417
Klebsiella pneumoniae DSM 2026
glycerone + NADH + H+
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Klebsiella pneumoniae
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
additional information
-
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
Klebsiella pneumoniae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
metal ion-chelated polyethyleneimine-immobilized enzyme PEI-Mn2+-GDH exhibits greater heat resistance than free enzyme GDH. After incubation for 90 min at 70°C, PEI-Mn2+-GDH still presents nearly 50% of its initial activity, while free GDH is almost totally inactivated
Klebsiella pneumoniae
85
-
metal ion-chelated polyethyleneimine-immobilized enzyme PEI-Mn2+-GDH exhibits a half-life that is enhanced by 5.6folds in aqueous phase at 85°C
Klebsiella pneumoniae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.06
-
glycerol
pH 10.0, 30°C, free enzyme
Klebsiella pneumoniae
3.36
-
glycerol
pH 10.0, 30°C, immobilized enzyme
Klebsiella pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
Klebsiella pneumoniae
10
11
the immobilized GDH changes the optimal pH from pH 11 to 10 and exhibits high activity in a relatively wide range of pH from pH 7 to 12, while free GDH only achieves high activity at pH 11
Klebsiella pneumoniae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
additional information
-
immobilization of the enzyme by metal ion-chelated polyethyleneimines greatly broadens the pH adaptability of GDH from strong alkaline into alkalescence
Klebsiella pneumoniae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
94
-
glycerol
pH 10.0, 30°C, free enzyme
Klebsiella pneumoniae
129
-
glycerol
pH 10.0, 30°C, immobilized enzyme
Klebsiella pneumoniae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
94
-
glycerol
pH 10.0, 30°C, free enzyme
Klebsiella pneumoniae
129
-
glycerol
pH 10.0, 30°C, immobilized enzyme
Klebsiella pneumoniae
Other publictions for EC 1.1.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748573
Patil
Fermentation of glycerol by A ...
Anaerobium acetethylicum
Microb. Biotechnol.
10
203-217
2017
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
740197
Mahadevan
An improved glycerol biosensor ...
Cellulomonas sp.
Biosens. Bioelectron.
92
417-424
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
740228
Zhang
Conversion of glycerol to 1,3- ...
Escherichia coli
Biotechnol. Lett.
38
1559-1564
2016
-
1
1
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
740856
Wang
Characterization of glycerol d ...
Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium thermosaccharolyticum DSM 571
J. Microbiol. Biotechnol.
26
1077-1086
2016
-
-
1
-
-
-
4
3
-
4
1
2
-
3
-
-
1
-
-
-
-
-
6
1
1
-
4
2
-
2
-
3
-
-
-
-
-
1
3
-
-
-
-
4
-
3
-
4
1
2
-
-
-
1
-
-
-
-
6
1
1
-
4
2
-
2
-
-
-
3
3
-
2
2
741417
Zhang
Bioinspired immobilization of ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Sci. Rep.
6
24163
2016
1
-
1
-
1
-
6
3
-
3
-
2
-
3
-
-
1
-
-
-
-
-
2
-
1
1
2
2
2
-
1
2
-
-
-
1
-
1
2
-
1
-
-
6
-
3
-
3
-
2
-
-
-
1
-
-
-
-
2
-
1
1
2
2
2
-
1
-
-
-
-
-
2
2
739908
Liu
Improved production of propion ...
Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. pneumoniae ATCC 12657
Appl. Environ. Microbiol.
81
2256-2264
2015
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
741239
Fang
Codon-optimized NADH oxidase g ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
PLoS ONE
10
e0128412
2015
-
1
1
-
1
-
-
2
-
-
-
2
-
5
-
-
1
-
-
-
1
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
739811
Musille
Structure of glycerol dehydrog ...
Serratia sp.
Acta Crystallogr. Sect. F
70
166-172
2014
-
-
1
1
-
-
-
-
-
1
2
1
-
4
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739941
Beauchamp
Characterization of Thermotoga ...
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
Appl. Microbiol. Biotechnol.
98
7039-7050
2014
-
2
1
-
9
-
-
23
-
-
-
2
-
4
-
-
1
-
-
-
1
-
6
-
1
1
2
-
1
2
-
3
-
-
-
-
2
1
3
-
9
-
-
-
-
23
-
-
-
2
-
-
-
1
-
-
1
-
6
-
1
1
2
-
1
2
-
-
-
-
-
-
-
-
740827
Zhang
Cloning, expression and charac ...
Serratia marcescens, Serratia marcescens H30
J. Ind. Microbiol. Biotechnol.
41
1319-1327
2014
-
-
1
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
-
-
-
2
1
1
1
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
741311
Fang
Mechanistic study of manganese ...
Klebsiella pneumoniae
PLoS ONE
9
e99162
2014
-
-
1
-
-
-
4
1
-
3
-
2
-
3
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
2
1
-
-
-
-
2
3
-
-
-
-
6
1
2
-
6
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
3
-
-
-
723679
Piattoni
-
Production and characterizatio ...
Escherichia coli
Process Biochem.
48
406-412
2013
-
-
1
-
-
-
-
13
-
2
3
1
-
1
-
-
1
-
-
-
-
-
2
1
1
1
-
-
2
2
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
13
-
2
3
1
-
-
-
1
-
-
-
-
2
1
1
1
-
-
2
2
-
-
-
3
3
-
-
-
740215
Wang
-
The improvement of stability, ...
Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. pneumoniae ATCC 700721
Biotechnol. Bioprocess Eng.
18
796-800
2013
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4
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1
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4
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1
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3
3
710902
Camacho Morales
Analysis of glycerol dehydroge ...
Mucor circinelloides, Mucor circinelloides YR-1
Antonie van Leeuwenhoek
98
437-445
2010
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7
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4
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1
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1
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1
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710976
Matsuzawa
The gld1+ gene encoding glycer ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe ARC039
Appl. Microbiol. Biotechnol.
87
715-727
2010
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2
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2
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1
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1
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-
710980
Zhang
Engineering of glycerol dehydr ...
Escherichia coli, Klebsiella pneumoniae, Klebsiella pneumoniae MGH78578, Salmonella enterica, Salmonella enterica ATCC 9150
Appl. Microbiol. Biotechnol.
88
117-124
2010
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3
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6
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10
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1
3
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9
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3
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9
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9
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5
3
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6
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10
10
735530
Yao
Metabolic engineering to impro ...
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
Appl. Microbiol. Biotechnol.
88
199-208
2010
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1
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-
747295
Raghava
Purification and characteriza ...
Thermus thermophilus, Thermus thermophilus MTCC 1494
Biores. Technol.
101
2554-2557
2010
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14
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1
2
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2
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1
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14
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1
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17
1
1
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1
-
1
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-
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-
695386
Goncalves
Crystallization and preliminar ...
Salmonella enterica subsp. enterica serovar Typhimurium
Acta Crystallogr. Sect. F
65
698-701
2009
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-
1
1
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2
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5
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1
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2
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1
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2
-
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-
695703
Dominguez
Sequential Injection Kinetic F ...
Cellulomonas sp.
Appl. Biochem. Biotechnol.
160
1664-1673
2009
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2
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1
1
-
1
-
-
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1
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-
-
-
695835
Hatayama
Regulation of glycerol metabol ...
Klebsiella aerogenes, Klebsiella aerogenes NBRC12010
Appl. Microbiol. Biotechnol.
83
749-756
2009
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4
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1
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-
-
696785
Zhao
Effects of over-expression of ...
Klebsiella pneumoniae, Klebsiella pneumoniae KG1
Bioprocess Biosyst. Eng.
32
313-320
2009
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1
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4
-
3
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-
696879
Durnin
Understanding and harnessing t ...
Escherichia coli
Biotechnol. Bioeng.
103
148-161
2009
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3
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1
-
1
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-
-
696915
Mouri
Design of a cytochrome P450BM3 ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
25
1372-1378
2009
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1
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-
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1
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32
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1
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1
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4
1
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1
1
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1
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1
1
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1
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1
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1
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4
1
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1
1
-
-
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-
-
-
-
699149
Zhao
-
Over-expression of glycerol de ...
Klebsiella pneumoniae, Klebsiella pneumoniae KG1
J. Chem. Technol. Biotechnol.
84
626-632
2009
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1
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1
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2
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2
1
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1
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1
1
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1
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2
1
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-
699973
Zhang
Tentative identification of gl ...
Escherichia coli
Med. Microbiol. Immunol.
198
195-204
2009
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1
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4
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1
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1
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1
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1
1
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1
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1
1
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-
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-
684422
Gamella
Integrated multienzyme electro ...
Cellulomonas sp.
Anal. Chim. Acta
609
201-209
2008
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1
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-
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-
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699986
Gonzalez
A new model for the anaerobic ...
Escherichia coli K-12
Metab. Eng.
10
234-245
2008
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1
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4
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1
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1
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1
1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
699987
Shams Yazdani
Engineering Escherichia coli f ...
Escherichia coli K-12
Metab. Eng.
10
340-351
2008
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-
1
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-
-
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2
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-
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-
-
-
-
-
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-
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684616
Gaetgens
Biotransformation of glycerol ...
Gluconobacter oxydans
Appl. Microbiol. Biotechnol.
76
553-559
2007
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1
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-
-
-
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-
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5
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-
-
-
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671323
Katrlik
-
Amperometric biosensors based ...
Cellulomonas sp.
Anal. Chim. Acta
566
11-18
2006
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1
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-
-
-
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-
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1
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1
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1
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1
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674122
Oliveira
Kinetic enzymatic determinatio ...
Cellulomonas sp., Klebsiella aerogenes
J. Agric. Food Chem.
54
4136-4140
2006
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2
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-
-
-
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2
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2
-
-
2
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2
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1
1
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-
1
1
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2
-
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2
-
2
-
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
674272
Nakayama
Mechanism of hilA repression b ...
Salmonella enterica
J. Bacteriol.
188
3121-3125
2006
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-
-
-
-
-
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2
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1
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1
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1
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-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
675794
Lee
Application of sequential inte ...
Escherichia coli
Metab. Eng.
8
58-65
2006
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1
1
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-
-
-
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-
-
-
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1
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-
1
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-
1
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668061
Ichinose
Enzymatic redox cofactor regen ...
Escherichia coli, Escherichia coli JM109
Biotechnol. Prog.
21
1192-1197
2005
3
1
1
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-
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1
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2
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31
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1
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-
1
1
-
4
-
1
-
-
-
3
1
-
3
-
-
-
3
1
1
3
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
1
1
-
4
-
1
-
-
-
3
1
-
-
-
-
-
-
-
-
655371
Stibor
-
Characterization of cold-activ ...
Arthrobacter sp.
Enzyme Microb. Technol.
32
532-538
2003
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
1
1
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
4
-
1
1
1
-
1
-
-
-
-
-
-
-
-
-
657315
Brinen
Crystal structure of a zinc-co ...
Thermotoga maritima, Thermotoga maritima TM0423
Proteins
50
371-374
2003
-
-
-
1
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
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-
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-
-
-
2
-
-
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-
-
-
2
1
-
-
-
-
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-
-
1
-
-
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-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654038
Yamada-Onodera
-
Characterisation of glycerol d ...
Ogataea angusta, Ogataea angusta DL-1
Acta Biotechnol.
22
337-353
2002
-
-
-
-
-
-
6
2
-
1
2
-
-
6
-
-
1
-
-
-
-
-
10
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
6
-
2
-
1
2
-
-
-
-
1
-
-
-
-
10
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654039
Yamada-Onodera
-
Expression of the gene of glyc ...
Ogataea angusta, Ogataea angusta DL-1
Acta Biotechnol.
22
355-362
2002
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654068
Srinivasan
Towards the crystal structure ...
Thermotoga maritima
Acta Crystallogr. Sect. D
58
867-869
2002
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656383
Pandey
Chemical modification of speci ...
Klebsiella aerogenes
J. Enzyme Inhib. Med. Chem.
17
49-53
2002
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
636297
Malaoui
Separation and characterizatio ...
Clostridium butyricum, Clostridium butyricum E5
J. Appl. Microbiol.
90
1006-1014
2001
-
-
-
-
-
-
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8
1
3
1
-
-
5
-
-
1
-
-
1
-
-
11
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
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8
1
3
1
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1
-
1
-
-
11
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
636300
Liyanage
Clostridium beijerinckii and C ...
Clostridioides difficile, Clostridium beijerinckii
Appl. Environ. Microbiol.
67
2004-2010
2001
-
-
-
-
-
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2
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7
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2
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2
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2
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-
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636301
Burke
Purification, crystallisation ...
Geobacillus stearothermophilus
Acta Crystallogr. Sect. D
57
165-167
2001
-
-
-
1
1
-
-
-
1
1
1
-
-
2
-
-
1
-
-
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1
1
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1
1
1
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1
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-
-
-
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-
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-
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-
-
137617
Altaras
Metabolic engineering of a 1,2 ...
Escherichia coli
Appl. Environ. Microbiol.
65
1180-1185
1999
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-
-
-
-
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1
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3
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1
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1
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1
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636298
Leichus
Isotopic analysis of the react ...
Cellulomonas sp.
Biochemistry
33
14642-14649
1994
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-
-
-
-
-
2
8
1
1
2
1
-
1
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-
1
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9
1
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2
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2
-
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2
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8
1
1
2
1
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1
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9
1
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636296
Nishise
-
Effects of organic solvents an ...
Cellulomonas sp., Cellulomonas sp. NT3060
Agric. Biol. Chem.
51
3347-3353
1987
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-
-
-
25
3
1
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2
-
4
-
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1
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6
-
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2
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2
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25
-
3
1
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2
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1
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6
-
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-
-
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-
587013
Kong
-
Glycerol oxidation and triose ...
Schizosaccharomyces pombe, Schizosaccharomyces pombe 972h-
J. Gen. Microbiol.
131
1571-1579
1985
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-
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1
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2
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2
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1
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2
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1
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2
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2
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1
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2
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1
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2
-
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1
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-
636283
Marshall
-
Purification and properties of ...
Schizosaccharomyces pombe
J. Gen. Microbiol.
131
1581-1588
1985
4
-
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-
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7
4
1
4
2
1
-
1
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-
1
-
-
-
1
2
9
1
-
-
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-
2
2
-
2
-
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-
4
-
-
2
-
-
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7
-
4
1
4
2
1
-
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-
1
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-
1
2
9
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
636285
Gärtner
-
Purification and properties of ...
Pichia membranifaciens, Pichia membranifaciens H 112
J. Gen. Microbiol.
130
3225-3233
1984
3
-
-
-
-
-
11
4
1
-
2
2
-
2
-
-
1
-
-
-
1
1
6
1
1
-
-
-
3
-
1
2
2
-
4
3
-
-
2
-
-
-
4
11
2
4
1
-
2
2
-
-
-
1
-
-
1
1
6
1
1
-
-
-
3
-
1
-
-
-
-
-
-
-
636286
Nishise
-
Further characterization of gl ...
Cellulomonas sp., Cellulomonas sp. NT3060
Agric. Biol. Chem.
48
1603-1609
1984
1
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1
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3
2
1
1
1
2
-
4
-
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1
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1
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8
1
2
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-
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4
-
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2
2
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1
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-
2
1
-
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3
2
2
1
1
1
2
-
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1
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1
-
8
1
2
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-
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4
-
-
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-
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-
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636287
Scharschmidt
-
Isolierung und Charakterisieru ...
Bacillus megaterium
Hoppe-Seyler's Z. Physiol. Chem.
364
911-921
1983
-
-
-
-
-
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8
2
1
1
2
1
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1
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1
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1
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3
1
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1
-
1
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2
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2
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8
-
2
1
1
2
1
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1
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1
-
3
1
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1
-
1
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-
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636288
Sasaki
Specific affinity of glycerol ...
Geotrichum candidum
J. Biochem.
91
211-217
1982
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4
-
1
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2
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1
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1
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4
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1
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1
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1
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-
636289
Yamada H.; Nagao
-
Glycerol dehydrogenase from Ce ...
Cellulomonas sp., Cellulomonas sp. NT3060
Agric. Biol. Chem.
46
2333-2339
1982
1
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9
3
1
4
-
2
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4
-
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1
-
-
-
1
-
17
-
1
-
2
-
2
2
1
2
-
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1
-
-
2
-
-
-
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9
-
3
1
4
-
2
-
-
-
1
-
-
1
-
17
-
1
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2
-
2
2
1
-
-
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-
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636290
Yamada
-
Crystallisation of glycerol de ...
Cellulomonas sp.
Agric. Biol. Chem.
44
471-472
1980
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
1
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636291
Ruch
In vivo inactivation of glycer ...
Klebsiella aerogenes 2103, Klebsiella aerogenes
J. Bacteriol.
141
1077-1085
1980
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1
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2
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1
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1
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2
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1
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2
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1
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1
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2
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636292
McGregor
Purification and kinetic chara ...
Klebsiella aerogenes 1033, Klebsiella aerogenes
J. Biol. Chem.
249
3132-3139
1974
5
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6
1
1
3
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3
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1
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1
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2
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5
-
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6
-
1
1
3
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1
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1
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2
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Lin
The activation of glycerol deh ...
Klebsiella aerogenes, Klebsiella aerogenes P14
J. Biol. Chem.
235
1820-1823
1960
1
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5
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1
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3
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1
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1
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10
-
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1
1
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2
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1
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2
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5
-
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1
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2
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1
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1
-
10
-
-
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1
1
-
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636294
Burton
-
Glycerol dehydrogenase from Ae ...
Klebsiella aerogenes
Methods Enzymol.
1
397-400
1955
1
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-
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-
4
1
-
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1
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1
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1
-
-
-
1
1
17
-
-
-
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1
3
2
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1
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2
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-
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4
1
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1
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1
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1
1
17
-
-
-
-
-
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1
3
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636295
Asnis
A glycerol dehydrogenase from ...
Escherichia coli, Escherichia coli ECFS
J. Biol. Chem.
203
153-159
1953
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-
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4
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1
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3
-
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1
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1
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1
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1
1
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4
-
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1
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1
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1
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1
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1
1
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