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Literature summary for 1.1.1.6 extracted from
- Purification and characterization of an alcohol dehydrogenase with an unusual specificity towards glycerol from Thermus thermophilus (2010), Biores. Technol., 101, 2554-2557 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
NADH | pH and temperature not specified in the publication | Thermus thermophilus |  |
| 0.015 | - |
NAD+ | pH and temperature not specified in the publication | Thermus thermophilus | |
| 0.07 | - |
D-glyceraldehyde | pH and temperature not specified in the publication | Thermus thermophilus | |
| 0.2 | - |
glycerol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 0.3 | - |
2-butanol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 3 | - |
1-phenylethanol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 4 | - |
2-propanol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 35 | - |
ethanol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 46 | - |
1-propanol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 56 | - |
ethylene glycol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 71 | - |
1,4-Butanediol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 76 | - |
benzyl alcohol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 840 | - |
1-butanol | pH and temperature not specified in the publication | Thermus thermophilus | |
| 840 | - |
1-Octanol | pH and temperature not specified in the publication | Thermus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | contains Zn2+ | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Thermus thermophilus |
| 170000 | - |
analytical ultracentrifugation | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus MTCC 1494 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified with 25fold purification and 68% yield using a single chromatographic step | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.5 | - |
pH and temperature not specified in the publication | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-butanediol + NAD+ | - |
Thermus thermophilus | ? + NADH + H+ | - |
? | |
| 1-butanol + NAD+ | - |
Thermus thermophilus | butanal + NADH + H+ | - |
? | |
| 1-butanol + NAD+ | - |
Thermus thermophilus MTCC 1494 | butanal + NADH + H+ | - |
? | |
| 1-octanol + NAD+ | - |
Thermus thermophilus | octanal + NADH + H+ | - |
? | |
| 1-octanol + NAD+ | - |
Thermus thermophilus MTCC 1494 | octanal + NADH + H+ | - |
? | |
| 1-phenylethanol + NAD+ | - |
Thermus thermophilus | acetophenone + NADH + H+ | - |
? | |
| 1-propanol + NAD+ | - |
Thermus thermophilus | propanal + NADH + H+ | - |
? | |
| 1-propanol + NAD+ | - |
Thermus thermophilus MTCC 1494 | propanal + NADH + H+ | - |
? | |
| 2-butanol + NAD+ | - |
Thermus thermophilus | 2-butanone + NADH + H+ | - |
? | |
| 2-propanol + NAD+ | - |
Thermus thermophilus | acetone + NADH + H+ | - |
? | |
| 2-propanol + NAD+ | - |
Thermus thermophilus MTCC 1494 | acetone + NADH + H+ | - |
? | |
| benzyl alcohol + NAD+ | - |
Thermus thermophilus | benzaldehyde + NADH + H+ | - |
? | |
| D-glyceraldehyde + NADH + H+ | - |
Thermus thermophilus | glycerol + NAD+ | - |
r | |
| ethanol + NAD+ | - |
Thermus thermophilus | acetaldehyde + NADH + H+ | - |
? | |
| ethylene glycol + NAD+ | - |
Thermus thermophilus | ? + NADH + H+ | - |
? | |
| glycerol + NAD+ | no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde | Thermus thermophilus | D-glyceraldehyde + NADH + H+ | - |
r | |
| glycerol + NAD+ | no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde | Thermus thermophilus MTCC 1494 | D-glyceraldehyde + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 43000, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TTHADH | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
glycerol oxidation | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 89 | - |
Tm-value | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
glycerol oxidation | Thermus thermophilus |
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