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Literature summary for 1.1.1.6 extracted from
- Biochemical and molecular characterization of glycerol dehydrogenase from Klebsiella pneumoniae (2020), J. Microbiol. Biotechnol., 30, 271-278 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta2(DE3) cells | Klebsiella pneumoniae subsp. pneumoniae |
| expression in Escherichia coli | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Tris HCl | Tris buffer lowers the enzyme activity about 7fold in comparison to CHES buffer | Klebsiella pneumoniae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
NAD+ | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 2.6 | - |
NAD+ | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 67.3 | - |
butane-2,3-diol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 67.3 | - |
racemic 2,3-butanediol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 91 | - |
glycerol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 91 | - |
glycerol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 92.7 | - |
ethylene glycol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 92.7 | - |
ethylene glycol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 370000 | - |
gel filtration | Klebsiella pneumoniae subsp. pneumoniae |
| 370000 | - |
and 710000, gel filtration | Klebsiella pneumoniae |
| 710000 | - |
and 370000, gel filtration | Klebsiella pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | Klebsiella pneumoniae subsp. pneumoniae | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NADP+ | Klebsiella pneumoniae subsp. pneumoniae | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | dihydroxyacetone + NADPH + H+ | - |
? | |
| glycerol + NADP+ | Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | dihydroxyacetone + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | A6TGD6 | - |
- |
| Klebsiella pneumoniae ATCC 700721 | A6TGD6 | - |
- |
| Klebsiella pneumoniae subsp. pneumoniae | A6TGD6 | - |
- |
| Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | A6TGD6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superdex 200 gel filtration | Klebsiella pneumoniae subsp. pneumoniae |
| recombinant protein | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.78 | - |
with NADP+ as cosubstrate, at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae |
| 101.68 | - |
pH 7.4, 35°C | Klebsiella pneumoniae |
| 101.68 | - |
with NAD+ as cosubstrate, at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae | ? + NADH + H+ | - |
? | |
| butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae ATCC 700721 | ? + NADH + H+ | - |
? | |
| ethylene glycol + NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae | ? | - |
? | |
| ethylene glycol + NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | ? | - |
? | |
| ethylene glycol + NAD+ | - |
Klebsiella pneumoniae | ? + NADH + H+ | - |
? | |
| ethylene glycol + NAD+ | - |
Klebsiella pneumoniae ATCC 700721 | ? + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Klebsiella pneumoniae | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae subsp. pneumoniae | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Klebsiella pneumoniae ATCC 700721 | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NADP+ | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae subsp. pneumoniae | dihydroxyacetone + NADPH + H+ | - |
? | |
| glycerol + NADP+ | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | dihydroxyacetone + NADPH + H+ | - |
? | |
| racemic 2,3-butanediol + NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae | 3-hydroxybutane-2-one + NADH + H+ | - |
? | |
| racemic 2,3-butanediol + NAD+ | - |
Klebsiella pneumoniae subsp. pneumoniae MGH78578 ATCC 700721 | 3-hydroxybutane-2-one + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexadecamer | 16 * 39800, calculated from sequence. Enzyme exist in an equilibrium between two different oligomeric states, octamer and hexadecamer | Klebsiella pneumoniae |
| homooctamer | 8 * 39800, calculated from amino acid sequence | Klebsiella pneumoniae subsp. pneumoniae |
| octamer | 8 * 39800, calculated fromsequence. Enzyme exist in an equilibrium between two different oligomeric states, octamer and hexadecamer | Klebsiella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GldA | - |
Klebsiella pneumoniae |
| GldA | - |
Klebsiella pneumoniae subsp. pneumoniae |
| GlyDH | - |
Klebsiella pneumoniae subsp. pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Klebsiella pneumoniae |
| 55 | 60 | - |
Klebsiella pneumoniae subsp. pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 83.4 | - |
melting temperature | Klebsiella pneumoniae subsp. pneumoniae |
| 83.4 | - |
melting temperature | Klebsiella pneumoniae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
ethylene glycol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 2.8 | - |
ethylene glycol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 22.5 | - |
butane-2,3-diol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 22.5 | - |
racemic 2,3-butanediol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 70.6 | - |
NAD+ | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 70.6 | - |
NAD+ | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 87.1 | - |
glycerol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 87.1 | - |
glycerol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
maximum activity at pH 8.6 in CHES buffer and pH 10.0 in CAPS buffer | Klebsiella pneumoniae |
| 8.6 | - |
the enzyme has two pH optima at pH 8.6 in CHES buffer (78% activity) and 10.0 in CAPS buffer (100% activity) | Klebsiella pneumoniae subsp. pneumoniae |
| 10 | - |
maximum activity at pH 8.6 in CHES buffer and pH 10.0 in CAPS buffer | Klebsiella pneumoniae |
| 10 | - |
the enzyme has two pH optima at pH 8.6 in CHES buffer (78% activity) and 10.0 in CAPS buffer (100% activity) | Klebsiella pneumoniae subsp. pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae | |
| NAD+ | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae subsp. pneumoniae | |
| NADP+ | about 2% of the activity with NAD+ | Klebsiella pneumoniae | |
| NADP+ | the enzyme exhibits an exclusive preference for NAD+ over NADP+ | Klebsiella pneumoniae subsp. pneumoniae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
ethylene glycol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 0.03 | - |
ethylene glycol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 0.33 | - |
racemic 2,3-butanediol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 0.33 | - |
butane-2,3-diol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 0.96 | - |
glycerol | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 0.96 | - |
glycerol | pH 7.4, 35°C | Klebsiella pneumoniae | |
| 27 | - |
NAD+ | at pH 8.6 and 35°C | Klebsiella pneumoniae subsp. pneumoniae | |
| 27 | - |
NAD+ | pH 7.4, 35°C | Klebsiella pneumoniae | |
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