Literature summary for 1.1.1.60 extracted from

Osipiuk, J.; Zhou, M.; Moy, S.; Collart, F.; Joachimiak, A.
X-ray crystal structure of GarR - tartronate semialdehyde reductase from Salmonella typhimurium (2009), J. Struct. Funct. Genomics, 10, 249-253.

Cloned(Commentary)

Cloned (Comment)	Organism
gene garR, expression of the His6-tagged enzyme in Escherichia coli strain BL21	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16°C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Comment)

Organism

enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16°C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method

Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
tartronate semialdehyde + NADH	Salmonella enterica subsp. enterica serovar Typhimurium	as cofactor in the final stage of D-glycerate biosynthesis	(R)-glycerate + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	gene garR	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged GarR from Escherichia coli strain BL21, the tag is removed by TEV protease, purification by nickel affinity chromatography	Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
tartronate semialdehyde + NADH	as cofactor in the final stage of D-glycerate biosynthesis	Salmonella enterica subsp. enterica serovar Typhimurium	(R)-glycerate + NAD+	-	?
tartronate semialdehyde + NADH + H+	-	Salmonella enterica subsp. enterica serovar Typhimurium	(R)-glycerate + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
GarR	-	Salmonella enterica subsp. enterica serovar Typhimurium
More	the enzyme belongs to family of structurally and mechanically related beta-hydroxyacid dehydrogenases, COG2084	Salmonella enterica subsp. enterica serovar Typhimurium
tartronate semialdehyde reductase	-	Salmonella enterica subsp. enterica serovar Typhimurium
TSR	-	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Salmonella enterica subsp. enterica serovar Typhimurium