Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ustilago maydis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | severe inhibition | Ustilago maydis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00019 | - |
2-hydroxy-3-oxopropanoate | pH 8.5, 40°C | Ustilago maydis | |
0.0003 | - |
NADP+ | pH 8.5, 40°C | Ustilago maydis | |
0.0143 | - |
NADH | pH 8.5, 40°C | Ustilago maydis | |
0.0177 | - |
D-glycerate | pH 8.5, 40°C | Ustilago maydis | |
0.123 | - |
L-Glycerate | pH 8.5, 40°C | Ustilago maydis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein | Ustilago maydis |
41000 | - |
2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein | Ustilago maydis |
85000 | - |
gel filtration | Ustilago maydis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ustilago maydis | A0A0D1DYW4 | putative | - |
Storage Stability | Organism |
---|---|
4°C, phosphate buffer, pH 7.0, improved solubility | Ustilago maydis |
4°C, Tris-HCl, pH 8.0, highly unstable, precipitates during storage | Ustilago maydis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-3-oxopropanoate + NADH + H+ | - |
Ustilago maydis | D-glycerate + NAD+ | - |
? | |
D-glycerate + NADP+ | enzyme shows strong specificity and enantioselectivity for D-glycerate | Ustilago maydis | 2-hydroxy-3-oxopropanoate + NADPH + H+ | - |
? | |
L-glycerate + NADP+ | enzyme shows strong specificity and enantioselectivity for D-glycerate | Ustilago maydis | 2-hydroxy-3-oxopropanoate + NADPH + H+ | - |
? | |
additional information | poor activity towards 6-phosphogluconic acid, beta-hydroxybutyric acid and D-threonine | Ustilago maydis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE, 2 * 41000, calculated, recombinant protein | Ustilago maydis |
Synonyms | Comment | Organism |
---|---|---|
TSR1 | - |
Ustilago maydis |
UM02592 | - |
Ustilago maydis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Ustilago maydis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Ustilago maydis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
rapidly precipitates below | Ustilago maydis |
7 | - |
phosphate buffer, pH 7.0, improved solubility | Ustilago maydis |
8 | - |
Tris-HCl, highly unstable, precipitates during storage | Ustilago maydis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | about 75% of the activity with NADP+ | Ustilago maydis | |
NADP+ | preferred over NAD+ | Ustilago maydis |
General Information | Comment | Organism |
---|---|---|
physiological function | over-expression of the gene results in improved glycerol assimilation. Glycolipid accumulation is reduced by 45.2% in the knockout mutant whereas over-expression of the gene increases it by 40.4% | Ustilago maydis |