Protein Variants | Comment | Organism |
---|---|---|
R80I | mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 80fold higher than the KM-value for the wild-type enzyme | Homo sapiens |
R80K | conversion of androst-4-ene-3,17-dione to testosterone is reduced as compared to wild-type enzyme. The Km-value for androstenedione is 3.5fold higher than the KM-value for the wild-type enzyme | Homo sapiens |
R80L | conversion of androst-4-ene-3,17-dione to testosterone is strongly reduced as compared to wild-type enzyme. The Km-value for androstenedione is 21.6fold higher than the KM-value for the wild-type enzyme | Homo sapiens |
R80M | mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 90.7fold higher than the KM-value for the wild-type enzyme | Homo sapiens |
R80Q | mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 61.5fold higher than the KM-value for the wild-type enzyme | Homo sapiens |
R80Y | mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 85.1fold higher than the KM-value for the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the KM-values for R80 mutant enzymes are higher than the Km-value of the wild-type enzyme: for R80K 3.5fold, for R80L 21.6fold, for R80M 90.7fold, for R80Q 61.5fold, for R80Y 61.5fold, for R80Y 85.1fold, for R80I 80fold | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
androst-4-ene-3,17-dione + NADPH | Homo sapiens | the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency | testosterone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
androst-4-ene-3,17-dione + NADPH | - |
Homo sapiens | testosterone + NADP+ | - |
? | |
androst-4-ene-3,17-dione + NADPH | the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency | Homo sapiens | testosterone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
17beta-HSD-3 | - |
Homo sapiens |
17beta-hydroxysteroid dehydrogenase type 3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no activity with NADH | Homo sapiens |