Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
D-mannitol | recombinant protein | Pseudomonas fluorescens | |
25 | - |
D-fructose | recombinant protein | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
recombinant protein, gel filtration | Pseudomonas fluorescens |
54000 | - |
1 * 54000, SDS-PAGE, recombinant protein | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | - |
Pseudomonas fluorescens | D-fructose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54000, SDS-PAGE, recombinant protein | Pseudomonas fluorescens |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
NAD+ | recombinant protein | Pseudomonas fluorescens | |
54 | - |
NADH | recombinant protein | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
D-fructose reduction, recombinant protein | Pseudomonas fluorescens |
10 | - |
D-mannitol oxidation, recombinant protein | Pseudomonas fluorescens |