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Literature summary for 1.1.1.67 extracted from
- A zinc-containing mannitol-2-dehydrogenase from Leuconostoc pseudomesenteroides ATCC 12291: purification of the enzyme and cloning of the gene (2003), Arch. Microbiol., 179, 101-107.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Leuconostoc pseudomesenteroides |
General Stability
| General Stability | Organism |
|---|---|
| DTT stabilizes | Leuconostoc pseudomesenteroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | 50 mM, pH 5.4, 72% inhibition | Leuconostoc pseudomesenteroides |  |
| EDTA | 20 mM, complete inhibition, can be restored by 20 mM Zn2+, but not by Mn2+ or Mg2+ | Leuconostoc pseudomesenteroides | |
| imidazole | 175 mM,pH 7.0, 74% inhibition | Leuconostoc pseudomesenteroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
D-mannitol | pH 8.6, 30°C | Leuconostoc pseudomesenteroides | |
| 44 | - |
D-fructose | pH 5.4, 30°C | Leuconostoc pseudomesenteroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | cofactor | Leuconostoc pseudomesenteroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Leuconostoc pseudomesenteroides |
| 155000 | - |
gel filtration | Leuconostoc pseudomesenteroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc pseudomesenteroides | Q83VI5 | ATCC 12291 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leuconostoc pseudomesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 450 | - |
- |
Leuconostoc pseudomesenteroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or 20°C, enzyme loses approximately 60% of its specific activity after storage for 3 weeks in presence of 1 mM DTT, without DTT the same loss is observed after 2 days | Leuconostoc pseudomesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | - |
Leuconostoc pseudomesenteroides | D-mannitol + NAD+ | - |
r | |
| D-glucitol + NAD+ | 4% of the activity with D-mannitol | Leuconostoc pseudomesenteroides | ? | - |
? | |
| D-mannitol + NADP+ | 32% of the activity with NAD+ | Leuconostoc pseudomesenteroides | D-fructose + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Leuconostoc pseudomesenteroides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mannitol 2-dehydrogenase | - |
Leuconostoc pseudomesenteroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
reduction of D-fructose | Leuconostoc pseudomesenteroides |
| 8.6 | - |
oxidation of D-mannitol | Leuconostoc pseudomesenteroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Leuconostoc pseudomesenteroides | |
| NADH | - |
Leuconostoc pseudomesenteroides | |
| NADP+ | 32% of the activity with NAD+ | Leuconostoc pseudomesenteroides | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leuconostoc pseudomesenteroides | chromatofocusing | - |
4.2 |
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