Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
NADH | at 25°C, in 100 mM Tris/HCl buffer, pH 7.1 | Aspergillus fumigatus | |
0.15 | - |
NAD+ | at 25°C, in 100 mM glycine/NaOH buffer pH 10.0 | Aspergillus fumigatus | |
13 | - |
D-mannitol | at 25°C, in 100 mM glycine/NaOH buffer pH 10.0 | Aspergillus fumigatus | |
60 | - |
D-fructose | at 25°C, in 100 mM Tris/HCl buffer, pH 7.1 | Aspergillus fumigatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not require metals for activity | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WQY4 | - |
- |
Purification (Comment) | Organism |
---|---|
Co2+ chelating Sepharose column chromatography and Superdex 200 gel filtration | Aspergillus fumigatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
crude extract, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1 | Aspergillus fumigatus |
33 | - |
after 6.7fold purification, at 25°C, using 100 mM Tris-HCl buffer, pH 7.1 | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + NAD+ | - |
Aspergillus fumigatus | D-fructose + NADH + H+ | - |
r | |
D-mannitol + NADP+ | M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH | Aspergillus fumigatus | D-fructose + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
M2DH | - |
Aspergillus fumigatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on, M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH | Aspergillus fumigatus | |
NADP+ | M2DH is a much poorer enzyme when it employes NADP+ and NADPH as compared to NAD+ and NADH | Aspergillus fumigatus |