Literature summary for 1.1.1.67 extracted from

Haghighatian, M.; Mofid, M.R.; Nekouei, M.K.; Yaghmaei, P.; Tafreshi, A.H.
Isomalt production by cloning, purifying and expressing of the MDH gene from Pseudomonas fluorescens DSM 50106 in different strains of E. coli (2008), Pak. J. Biol. Sci., 11, 2001-2006.

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Application

Application	Comment	Organism
industry	best strain for expression of MDH in both laboratory and industrial applications is Escherichia coli BL21 (DE3) plysS	Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment)	Organism
subcloned into vector pDEST110 and overexpressed in different strains of Escherichia coli (BL21 (DE3) plysS, JM109, Origami(DE3) or M15)	Pseudomonas fluorescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, SDS-PAGE	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	DSM 50106	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni2+-NTA affinity chromatography	Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
63	-	MDH expressed in Escherichia coli strain BL21 (DE3) plysS, with fructose as substrate	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arabitol + NAD(P)+	-	Pseudomonas fluorescens	? + NAD(P)H	-	?
D-fructose + NAD(P)+	-	Pseudomonas fluorescens	? + NAD(P)H	-	?
isomaltulose + NAD(P)+	-	Pseudomonas fluorescens	? + NAD(P)H	-	?
additional information	among the Escherichia coli strains, BL21 (DE3) plysS exhibits the maximum expression level of MDH (11mg/L)	Pseudomonas fluorescens	?	-	?
sorbitol + NAD(P)+	-	Pseudomonas fluorescens	? + NAD(P)H	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, SDS-PAGE	Pseudomonas fluorescens

Synonyms

Synonyms	Comment	Organism
MDH	-	Pseudomonas fluorescens
NADH-dependent mannitol dehydrogenase	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)