Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, calculated from amino acid sequence | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | Sinorhizobium meliloti | - |
D-mannitol + NAD+ | - |
r | |
D-fructose + NADH + H+ | Sinorhizobium meliloti KCTC 2353 | - |
D-mannitol + NAD+ | - |
r | |
D-mannitol + NAD+ | Sinorhizobium meliloti | - |
D-fructose + NADH + H+ | - |
r | |
D-mannitol + NAD+ | Sinorhizobium meliloti KCTC 2353 | - |
D-fructose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
- |
- |
Sinorhizobium meliloti KCTC 2353 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | - |
Sinorhizobium meliloti | D-mannitol + NAD+ | - |
r | |
D-fructose + NADH + H+ | - |
Sinorhizobium meliloti KCTC 2353 | D-mannitol + NAD+ | - |
r | |
D-mannitol + NAD+ | - |
Sinorhizobium meliloti | D-fructose + NADH + H+ | - |
r | |
D-mannitol + NAD+ | - |
Sinorhizobium meliloti KCTC 2353 | D-fructose + NADH + H+ | - |
r | |
additional information | no activity with NADP+ and NADPH | Sinorhizobium meliloti | ? | - |
? | |
additional information | no activity with NADP+ and NADPH | Sinorhizobium meliloti KCTC 2353 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, calculated from amino acid sequence | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Sinorhizobium meliloti |
NAD+-dependent mannitol dehydrogenase | - |
Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
optimum pH for D-fructose reduction | Sinorhizobium meliloti |
10 | - |
optimum pH for D-mannitol oxidation | Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sinorhizobium meliloti |