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Literature summary for 1.1.1.71 extracted from
- NADP-dependent alcohol dehydrogenases in bacteria and yeast: purification and partial characterization of the enzymes from Acinetobacter calcoaceticus and Saccharomyces cerevisiae (1994), Microbiology, 140, 173-183.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 0.01 mM, complete inhibition | Acinetobacter calcoaceticus |  |
| 4-chloromercuribenzoate | 0.01 mM, complete inhibition; some protection of partial inhibition resulting from 0.001 mM 4-chloromercuribenzoate in the presence of 1 mM NADP+, almost complete protection with 10 mM hexan-1-ol | Saccharomyces cerevisiae | |
| EDTA | 10 mM, 20% inhibition | Saccharomyces cerevisiae | |
| iodoacetamide | 1 mM, complete inhibition | Saccharomyces cerevisiae | |
| N-ethylmaleimide | 1 mM, complete inhibition | Saccharomyces cerevisiae | |
| Sodium azide | 10 mM, 40% inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00079 | - |
NADPH | - |
Saccharomyces cerevisiae | |
| 0.00433 | - |
NADPH | - |
Acinetobacter calcoaceticus | |
| 0.0202 | - |
NADP+ | - |
Saccharomyces cerevisiae | |
| 0.038 | - |
NADP+ | - |
Acinetobacter calcoaceticus | |
| 0.75 | - |
Butanal | - |
Saccharomyces cerevisiae | |
| 1.09 | - |
heptan-1-ol | - |
Acinetobacter calcoaceticus | |
| 1.22 | - |
hexan-1-ol | - |
Acinetobacter calcoaceticus | |
| 3.97 | - |
Butanal | - |
Acinetobacter calcoaceticus | |
| 5.8 | - |
pentan-1-ol | - |
Acinetobacter calcoaceticus | |
| 6.2 | - |
octan-1-ol | - |
Saccharomyces cerevisiae | |
| 6.8 | - |
hexan-1-ol | - |
Saccharomyces cerevisiae | |
| 14.1 | - |
pentan-1-ol | - |
Saccharomyces cerevisiae | |
| 52.7 | - |
butan-1-ol | - |
Acinetobacter calcoaceticus | |
| 78.6 | - |
butan-1-ol | - |
Saccharomyces cerevisiae | |
| 150 | - |
benzyl alcohol | - |
Acinetobacter calcoaceticus | |
| 727 | - |
ethanol | - |
Acinetobacter calcoaceticus | |
| 4790 | - |
ethanol | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40300 | - |
4 * 40300, SDS-PAGE | Acinetobacter calcoaceticus |
| 43500 | - |
1 * 43500, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter calcoaceticus | - |
strains NCIB 8250 and HO1-N | - |
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis NCIMB 3610 | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Escherichia coli ML30 | - |
- |
- |
| Mycobacterium tuberculosis | - |
var. bosis | - |
| Pseudomonas aeruginosa | - |
strains NCIMB 10548 and 196A | - |
| Pseudomonas putida | - |
NCIMB 9494 | - |
| Pseudomonas putida NCIMB 9494 | - |
NCIMB 9494 | - |
| Rhodococcus rhodochrous | - |
- |
- |
| Rhodococcus rhodochrous NCIMB 13259 | - |
- |
- |
| Rhodotorula graminis | - |
- |
- |
| Rhodotorula graminis KGX 39 | - |
- |
- |
| Saccharomyces cerevisiae | - |
D273-10B | - |
| Saccharomyces cerevisiae D273-10B | - |
D273-10B | - |
| Streptomyces rimosus | - |
- |
- |
| Streptomyces rimosus 4018 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A | Saccharomyces cerevisiae |
| DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel | Acinetobacter calcoaceticus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+ | enzyme is stereospecific for the pro-R hydride of NADPH | Saccharomyces cerevisiae | |
| a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+ | enzyme is stereospecific for the pro-R hydride of NADPH | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allyl alcohol + NADP+ | 28% of activity with hexan-1-ol | Acinetobacter calcoaceticus | acrylaldehyde + NADPH | - |
r | |
| benzyl alcohol + NADP+ | - |
Acinetobacter calcoaceticus | benzaldehyde + NADPH | - |
r | |
| butan-1,4-diol + NADP+ | 29% of activity with hexan-1-ol | Acinetobacter calcoaceticus | butandialdehyde + NADPH | - |
r | |
| butan-1-ol + NADP+ | 36.3% of activity with hexan-1-ol | Acinetobacter calcoaceticus | butanal + NADPH | - |
r | |
| butan-1-ol + NADP+ | - |
Saccharomyces cerevisiae | butanal + NADPH + H+ | - |
r | |
| butan-1-ol + NADP+ | - |
Saccharomyces cerevisiae D273-10B | butanal + NADPH + H+ | - |
r | |
| butanal + NADPH + H+ | - |
Saccharomyces cerevisiae | butan-1-ol + NADP+ | - |
r | |
| butanal + NADPH + H+ | - |
Saccharomyces cerevisiae D273-10B | butan-1-ol + NADP+ | - |
r | |
| cis-hex-3-en-1-ol + NADP+ | 18.7% of activity with hexan-1ol | Acinetobacter calcoaceticus | cis-hex-3-en-1-al + NADPH | - |
r | |
| decan-1-ol + NADP+ | decrease in enzyme activity with increase in chain length | Acinetobacter calcoaceticus | decanal + NADPH | - |
r | |
| dodecan-1-ol + NADP+ | decrease in enzyme activity with increase in chain length | Acinetobacter calcoaceticus | dodecanal + NADPH | - |
r | |
| ethanediol + NADP+ | 8.5% of activity with hexan-1-ol | Acinetobacter calcoaceticus | glyoxal + NADPH | - |
r | |
| ethanol + NADP+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | - |
Saccharomyces cerevisiae D273-10B | acetaldehyde + NADPH + H+ | - |
r | |
| ethanol + NADP+ | 8.5% of activity with hexan-1-ol | Acinetobacter calcoaceticus | ethanal + NADPH + H+ | - |
r | |
| heptan-1-ol + NADP+ | - |
Acinetobacter calcoaceticus | heptanal + NADPH | - |
r | |
| hexan-1-ol + NADP+ | - |
Bacillus subtilis | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Escherichia coli | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Saccharomyces cerevisiae | hexanal + NADPH | - |
r | |
| hexan-1-ol + NADP+ | - |
Rhodococcus rhodochrous | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Pseudomonas aeruginosa | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Pseudomonas putida | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Acinetobacter calcoaceticus | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Acinetobacter calcoaceticus | hexanal + NADPH | - |
r | |
| hexan-1-ol + NADP+ | - |
Mycobacterium tuberculosis | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Rhodotorula graminis | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Streptomyces rimosus | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Streptomyces rimosus 4018 | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Rhodococcus rhodochrous NCIMB 13259 | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Pseudomonas putida NCIMB 9494 | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Rhodotorula graminis KGX 39 | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Saccharomyces cerevisiae D273-10B | hexanal + NADPH | - |
r | |
| hexan-1-ol + NADP+ | - |
Bacillus subtilis NCIMB 3610 | hexanal + NADPH | - |
? | |
| hexan-1-ol + NADP+ | - |
Escherichia coli ML30 | hexanal + NADPH | - |
? | |
| mannitol + NADP+ | 6.5% of activity with hexan-1-ol | Acinetobacter calcoaceticus | D-mannose + NADPH | - |
r | |
| octan-1-ol + NADP+ | decrease in enzyme activity with increase in chain length | Acinetobacter calcoaceticus | octanal + NADPH | - |
r | |
| octan-1-ol + NADP+ | - |
Saccharomyces cerevisiae | octanal + NADPH + H+ | - |
r | |
| pentan-1-ol + NADP+ | - |
Saccharomyces cerevisiae | pentanal + NADPH | - |
? | |
| pentan-1-ol + NADP+ | 4% of activity with hexan-1-ol | Acinetobacter calcoaceticus | pentanal + NADPH | - |
r | |
| pentan-1-ol + NADP+ | - |
Saccharomyces cerevisiae D273-10B | pentanal + NADPH | - |
? | |
| propan-1-ol + NADP+ | 28% of activity with hexan-1-ol | Acinetobacter calcoaceticus | propanal + NADPH | - |
r | |
| ribitol + NADP+ | 4% of activity with hexan-1-ol | Acinetobacter calcoaceticus | D-ribose + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43500, SDS-PAGE | Saccharomyces cerevisiae |
| tetramer | 4 * 40300, SDS-PAGE | Acinetobacter calcoaceticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13.9 | - |
ethanol | - |
Acinetobacter calcoaceticus | |
| 19.6 | - |
Butanal | - |
Saccharomyces cerevisiae | |
| 25.4 | - |
octan-1-ol | - |
Acinetobacter calcoaceticus | |
| 26.3 | - |
Butanal | - |
Acinetobacter calcoaceticus | |
| 35.1 | - |
ethanol | - |
Saccharomyces cerevisiae | |
| 38.3 | - |
butan-1-ol | - |
Acinetobacter calcoaceticus | |
| 38.9 | - |
heptan-1-ol | - |
Acinetobacter calcoaceticus | |
| 39.4 | - |
hexan-1-ol | - |
Acinetobacter calcoaceticus | |
| 39.8 | - |
octan-1-ol | - |
Saccharomyces cerevisiae | |
| 40.3 | - |
hexan-1-ol | - |
Saccharomyces cerevisiae | |
| 44.7 | - |
pentan-1-ol | - |
Acinetobacter calcoaceticus | |
| 50.6 | - |
butan-1-ol | - |
Saccharomyces cerevisiae | |
| 62.9 | - |
pentan-1-ol | - |
Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
hexanal reduction | Saccharomyces cerevisiae |
| 6.8 | - |
hexanal reduction | Acinetobacter calcoaceticus |
| 10.2 | - |
hexan-1-ol oxidation | Acinetobacter calcoaceticus |
| 10.7 | - |
hexan-1-ol oxidation | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 7.15 | hexanal reduction, 95% of maximal rate | Saccharomyces cerevisiae |
| 6.4 | 7.15 | hexanal reduction, 95% of maximal rate | Acinetobacter calcoaceticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Bacillus subtilis | |
| NADPH | - |
Escherichia coli | |
| NADPH | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Rhodococcus rhodochrous | |
| NADPH | - |
Pseudomonas aeruginosa | |
| NADPH | - |
Pseudomonas putida | |
| NADPH | - |
Acinetobacter calcoaceticus | |
| NADPH | - |
Mycobacterium tuberculosis | |
| NADPH | - |
Rhodotorula graminis | |
| NADPH | - |
Streptomyces rimosus | |
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