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Literature summary for 1.1.1.72 extracted from

  • Flynn, T.G.; Cromlish, J.A.
    Glycerol dehydrogenase from rabbit muscle (1982), Methods Enzymol., 89, 237-242.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Oryctolagus cuniculus
Diphenylhydantoin
-
Oryctolagus cuniculus
formononetin 7-O-glucoside
-
Oryctolagus cuniculus
Phenobarbitol
-
Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
186
-
D-glucuronate
-
Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde + NADPH + H+ Oryctolagus cuniculus
-
glycerol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.75
-
-
Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, dialyzed against distilled H2O with 30 mg/ml polyethyleneglycol, or lyophilized, stable Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrobenzaldehyde + NADPH
-
Oryctolagus cuniculus 4-nitrobenzylalcohol + NADP+
-
?
D-erythrose + NADPH
-
Oryctolagus cuniculus erythritol + NADP+
-
?
D-glucose + NADPH
-
Oryctolagus cuniculus sorbitol + NADP+
-
?
D-glucuronate + NADP+
-
Oryctolagus cuniculus ?
-
?
D-glyceraldehyde + NADPH + H+
-
Oryctolagus cuniculus glycerol + NADP+
-
r
D-xylose + NADPH
-
Oryctolagus cuniculus xylitol + NADP+
-
?
formaldehyde + NADPH
-
Oryctolagus cuniculus methanol + NADP+
-
?
glycolaldehyde + NADPH
-
Oryctolagus cuniculus ethyleneglycol + NADP+
-
?
L-glyceraldehyde + NADPH
-
Oryctolagus cuniculus glycerol + NADP+
-
?
methylglyoxal + NADPH
-
Oryctolagus cuniculus 1-hydroxyacetone + NADP+
-
?
n-butyraldehyde + NADPH
-
Oryctolagus cuniculus n-butanol + NADP+
-
?
n-pentanal + NADPH + H+
-
Oryctolagus cuniculus n-pentanol + NADP+
-
?
propionaldehyde + NADPH
-
Oryctolagus cuniculus propanol + NADP+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
DL-glyceraldehyde reduction Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NADH 10% of NADPH-activity Oryctolagus cuniculus
NADP+
-
Oryctolagus cuniculus
NADPH
-
Oryctolagus cuniculus