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Literature summary for 1.1.1.73 extracted from

  • Yamamoto, H.; Kudoh, M.
    Novel chiral tool, (R)-2-octanol dehydrogenase, from Pichia finlandica: Purification, gene cloning, and application for optically active alpha-haloalcohols (2013), Appl. Microbiol. Biotechnol., 97, 8087-8096.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 123% activity at 1 mM Ogataea wickerhamii
N-ethylmaleimide 147% activity at 1 mM Ogataea wickerhamii
o-phenanthroline 123% activity at 1 mM Ogataea wickerhamii
p-chloromercuribenzoate 118% activity at 0.05 mM Ogataea wickerhamii
phenylmethylsulfonyl fluoride 111% activity at 1 mM Ogataea wickerhamii
ZnSO4 152% activity at 1 mM Ogataea wickerhamii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Ogataea wickerhamii

Inhibitors

Inhibitors Comment Organism Structure
EDTA 80.6% residual activity at 1 mM Ogataea wickerhamii
Fe2+ 93.2% residual activity at 1 mM Ogataea wickerhamii
Hg+ 61.9% residual activity at 1 mM Ogataea wickerhamii
additional information not inhibited by Co2+, Zn2+, K+, Ni2+, Ba2+, NH2OH, and quercetin Ogataea wickerhamii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 174% activity at 1 mM Ogataea wickerhamii
Cu2+ 100% activity at 1 mM Ogataea wickerhamii
Mg2+ 162% activity at 1 mM Ogataea wickerhamii
Mn2+ 185% activity at 1 mM Ogataea wickerhamii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Ogataea wickerhamii
83000
-
gel filtration Ogataea wickerhamii

Organism

Organism UniProt Comment Textmining
Ogataea wickerhamii A0JC04
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, HiTrap Blue column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Ogataea wickerhamii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0224
-
crude extract, at pH 8.5 and 30°C Ogataea wickerhamii
91.1
-
after 4070fold purification, at pH 8.5 and 30°C Ogataea wickerhamii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R)-1-phenylethan-1-ol + NAD+ 255% activity compared to (R)-octan-2-ol Ogataea wickerhamii phenylacetaldehyde + NADH + H+
-
?
(1S)-1-phenylethan-1-ol + NAD+ 1.8% activity compared to (R)-octan-2-ol Ogataea wickerhamii phenylacetaldehyde + NADH + H+
-
?
(2R)-butan-2-ol + NAD+ 49.3% activity compared to (R)-octan-2-ol Ogataea wickerhamii butan-2-one + NADH + H+
-
?
(2R)-hexan-2-ol + NAD+ 54.1% activity compared to (R)-octan-2-ol Ogataea wickerhamii hexan-2-one + NADH + H+
-
?
(2S)-butan-2-ol + NAD+ 7.3% activity compared to (R)-octan-2-ol Ogataea wickerhamii butan-2-one + NADH + H+
-
?
(R)-octan-2-ol + NAD+ 100% activity Ogataea wickerhamii octan-2-one + NADH + H+
-
?
(RS)-octan-2-ol + NAD+ 77.8% activity compared to (R)-octan-2-ol Ogataea wickerhamii octan-2-one + NADH + H+
-
?
(S)-octan-2-ol + NAD+ 4.7% activity compared to (R)-octan-2-ol Ogataea wickerhamii octan-2-one + NADH + H+
-
?
1-phenoxypropan-2-ol + NAD+ 15.5% activity compared to (R)-octan-2-ol Ogataea wickerhamii 1-phenoxypropan-2-one + NADH + H+ +
-
?
1-phenoxypropan-2-one + NADH + H+ 54.2% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 1-phenoxypropan-2-ol + NAD+
-
?
1-phenylethan-1-one + NADH + H+ 45.3% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 1-phenylethan-1-ol + NAD+
-
?
2-bromo-1-cyclopropylethan-1-one + NADH + H+ 54.3% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-bromo-1-cyclopropylethan-1-ol + NAD+
-
?
2-chloro-1-(3-chlorophenyl)ethan-1-one + NADH + H+ 64.7% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-(3-chlorophenyl)ethan-1-ol + NAD+
-
?
2-chloro-1-(4-fluorophenyl)ethan-1-one + NADH + H+ 89.2% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-(4-fluorophenyl)ethan-1-ol + NAD+
-
?
2-chloro-1-(pyridin-3-yl)ethan-1-one + NADH + H+ 9.4% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-(pyridin-3-yl)ethan-1-ol + NAD+
-
?
2-chloro-1-phenylethan-1-one + NADH + H+ 52.6% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-phenylethan-1-ol + NAD+
-
?
3-acetyloxolan-2-one + NADH + H+ 12.7% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 3-acetyloxolan-2-ol + NAD+
-
?
5-chloropentan-2-one + NADH + H+ 108% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 5-chloropentan-2-ol + NAD+
-
?
butan-2-one + NADH + H+ 8.0% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii butan-2-ol + NAD+
-
?
ethanol + NAD+ 0.7% activity compared to (R)-octan-2-ol Ogataea wickerhamii acetaldehyde + NADH + H+
-
?
ethyl (3R)-3-hydroxybutanoate + NAD+ 41.1% activity compared to (R)-octan-2-ol Ogataea wickerhamii ethyl 3-oxobutanoate + NADH + H+
-
?
ethyl (3S)-3-hydroxybutanoate + NAD+ 1.0% activity compared to (R)-octan-2-ol Ogataea wickerhamii ethyl 3-oxobutanoate + NADH + H+
-
?
ethyl (3S)-4-chloro-3-hydroxybutanoate + NAD+ 2.2% activity compared to (R)-octan-2-ol Ogataea wickerhamii ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
?
ethyl 2-chloro-3-oxobutanoate + NADH + H+ 571% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii ethyl 2-chloro-3-oxobutanoate + NAD+
-
?
ethyl 3-oxobutanoate + NADH + H+ 105.8% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii ethyl 3-hydroxybutanoate + NAD+
-
?
ethyl 4-chloroacetoacetate + NADH + H+ 100% activity Ogataea wickerhamii ?
-
?
octan-1-ol + NAD+ 1.7% activity compared to (R)-octan-2-ol Ogataea wickerhamii octanal + NADH + H+
-
?
octan-2-one + NADH + H+ 31.2% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii octan-2-ol + NAD+
-
?
propan-2-ol + NAD+ 6.8% activity compared to (R)-octan-2-ol Ogataea wickerhamii propan-2-one + NADH + H+
-
?
propan-2-one + NADH + H+ 1.8% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii propan-2-ol + NAD+
-
?

Subunits

Subunits Comment Organism
homotrimer 2 * 30000, SDS-PAGE Ogataea wickerhamii

Synonyms

Synonyms Comment Organism
(R)-2-octanol dehydrogenase
-
Ogataea wickerhamii
ODH
-
Ogataea wickerhamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
oxidation reaction Ogataea wickerhamii
50 55 reduction reaction Ogataea wickerhamii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction reaction Ogataea wickerhamii
10.5
-
oxidation reaction Ogataea wickerhamii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ogataea wickerhamii