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Literature summary for 1.1.1.75 extracted from
- Amino ketone formation and aminopropanol-dehydrogenase activity in rat-liver preparations (1967), Biochem. J., 102, 511-519.
General Stability
| General Stability | Organism |
|---|---|
| stable on freezing and thawing an ultrasonication | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Rattus norvegicus |  |
| D,L-3-hydroxybutyrate | non-competitive, Ki: 200 mM in liver homogenates, little effect in soluble fraction from disintegrated mitochondria | Rattus norvegicus | |
| EDTA | weak inhibition at high concentration, little effect in soluble extract of disintegrated mitochondria | Rattus norvegicus | |
| L-cysteine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
1-aminopropan-2-ol | liver homogenates | Rattus norvegicus | |
| 200 | - |
D,L-4-amino-3-hydroxybutyrate | liver homogenates | Rattus norvegicus | |
| 200 | - |
1-aminopropan-2-ol | soluble extract of disintegrated mitochondria | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | inhibitory at all concentrations | Rattus norvegicus | |
| Mg2+ | slightly activating at 1.3 mM, inhibitory above 3 mM | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-1-aminopropan-2-ol + NAD+ | Rattus norvegicus | possible role in carnitine metabolism | aminoacetone + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| muscle | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | - |
soluble extract of disintegrated mitochondria | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D,L-1-aminopropan-2-ol + NAD+ | - |
Rattus norvegicus | aminoacetone + NADH | - |
? | |
| D,L-4-amino-3-hydroxybutyrate + NAD+ | - |
Rattus norvegicus | 4-amino-3-oxobutyrate + NADH | - |
? | |
| L-1-aminopropan-2-ol + NAD+ | - |
Rattus norvegicus | aminoacetone + NADH | - |
? | |
| L-1-aminopropan-2-ol + NAD+ | possible role in carnitine metabolism | Rattus norvegicus | aminoacetone + NADH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.1 | - |
soluble extract of disintegrated mitochondria | Rattus norvegicus |
| 9.8 | - |
liver homogenates | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 11 | very low activity at pH 7.5, 60% of activity at pH 11 in liver homogenates | Rattus norvegicus |
| 8.5 | 10.5 | 40% of activity in soluble extract of disintegrated mitochondria | Rattus norvegicus |
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Release 2023.1 (January 2023)
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