Literature summary for 1.1.1.76 extracted from

Guo, Z.; Zhao, X.; He, Y.; Yang, T.; Gao, H.; Li, G.; Chen, F.; Sun, M.; Lee, J.K.; Zhang, L.
Efficient (3R)-acetoin production from meso-2,3-butanediol using a new whole-cell biocatalyst with co-expression of meso-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin (2016), J. Microbiol. Biotechnol., 27, 92-100 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene mbdh, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)	Serratia sp. T241

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2S,3S)-butane-2,3-diol + NAD+	Serratia sp. T241	-	(S)-acetoin + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Serratia sp. T241	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.14	-	recombinant enzyme, pH 8.0, 22°C	Serratia sp. T241

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S,3S)-butane-2,3-diol + NAD+	-	Serratia sp. T241	(S)-acetoin + NADH + H+	-	?
additional information	meso-2,3-BDH from Serratia sp. T241 exhibits higher catalytic efficiency compared with the meso-2,3-BDHs from Klebsiella pneumoniae strain XJ-Li and Serratia marcescens strain H30. No activity is detected for (2R,3R)-2,3-BD as substrate by meso-2,3-BDH, but meso-2,3-BDH from Serratia sp. T241 can efficiently convert (2S,3S)-2,3-BD and meso-2,3-BD into (3S)-acetoin and (3R)-acetoin, respectively	Serratia sp. T241	?	-	-

Synonyms

Synonyms	Comment	Organism
(S,S)-2,3-BDH	-	Serratia sp. T241
mbdh	-	Serratia sp. T241
More	cf. EC 1.1.1.B20	Serratia sp. T241

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Serratia sp. T241

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Serratia sp. T241

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Serratia sp. T241

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Release 2023.1 (January 2023)