General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Rattus norvegicus |
ammonium sulfate stabilizes | Rattus norvegicus |
bovine serum albumin stabilizes | Rattus norvegicus |
lyophilization inactivates | Rattus norvegicus |
phosphate buffer stabilizes | Rattus norvegicus |
repeated freezing and thawing results in rapid loss of activity, to some extent restorable at room temperature | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | reversible by dithiothreitol, 10 mM inhibits to 50% | Rattus norvegicus | |
N-ethylmaleimide | reversible by dithiothreitol, 5 mM inhibits more than 90% of the enzyme activity | Rattus norvegicus | |
p-chloromercuribenzoate | 10 nM, strong inhibition; reversible by dithiothreitol, 0.001 mM inhibits more than 90% of the enzyme activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADH | less than 0.01 mM | Rattus norvegicus | |
0.03 | - |
NAD+ | - |
Rattus norvegicus | |
0.17 | - |
dihydroxyacetone phosphate | - |
Rattus norvegicus | |
0.3 | - |
alpha-glycerol phosphate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Rattus norvegicus |
75000 | - |
sucrose density gradient ultracentrifugation | Rattus norvegicus |
78000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Rattus norvegicus | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | Rattus norvegicus Sprague-Dawley | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Comment) | Organism |
---|---|
ion-exchange chromatography combined with affinity elution, 2 isozymes that differ in charge by analytical PAGE | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
152.4 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Rattus norvegicus | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
sn-glycerol 3-phosphate + NAD+ | - |
Rattus norvegicus Sprague-Dawley | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
at least 5 min stable | Rattus norvegicus |
50 | - |
rapid denaturation, prevented by 0.1 mg/ml bovine serum albumin, by over 20% ammonium sulfate or by 0.1 M phosphate buffer, pH 7.5 | Rattus norvegicus |
60 | - |
inactivation after 5 min | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
reduction of dihydroxyacetone phosphate, purified enzyme, decline in activity is more rapid at higher pH values, 7.3-7.5 crude homogenate of enzyme | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.2 | about half-maximal activity at pH 6.5 and 8.2, reduction of dihydroxyacetone phosphate | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
more stable at alkaline pH, more stable in phosphate buffer than in Tris-glycine buffer | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus |