General Stability | Organism |
---|---|
NADH stabilizes | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values of isozyme GPDH-3 | Drosophila melanogaster | |
0.078 | - |
NADH | GPDH-1 | Drosophila melanogaster | |
0.38 | - |
NAD+ | GPDH-1 | Drosophila melanogaster | |
0.52 | - |
dihydroxyacetone phosphate | GPDH-1 | Drosophila melanogaster | |
0.59 | - |
glycerol 3-phosphate | GPDH-1 | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Drosophila melanogaster | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
fruit fly, Samarkand wild-type | - |
Purification (Comment) | Organism |
---|---|
3 isozymes, product of the same gene mapped to left arm of chromosome II, isozyme 1: flight muscle, isozyme 3: larval and adult fat body, isozyme 2: heterodimer of 1 and 3 | Drosophila melanogaster |
affinity chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | thoracic flight muscle | Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
isozyme GPDH-3 | Drosophila melanogaster |
179.8 | - |
isozyme GPDH-1 | Drosophila melanogaster |
Storage Stability | Organism |
---|---|
5°C, isozyme 1: stable for over a month in 10 mM sodium phosphate buffer, pH 6.5, with added EDTA, dithiothreitol and NADH, isozyme 3: 50% loss of activity under the same conditions | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Drosophila melanogaster | dihydroxyacetone phosphate + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
reduction of dihydroxyacetone-P, isozyme GPDH-1 | Drosophila melanogaster |
7.4 | - |
reduction of dihydroxyacetone-phosphate, isozyme GPDH-3 | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Drosophila melanogaster | |
NADH | - |
Drosophila melanogaster |