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Literature summary for 1.1.1.8 extracted from

  • Cai, M.; He, L.H.; Yu, T.Y.
    Molecular clone and expression of a NAD+-dependent glycerol-3-phosphate dehydrogenase isozyme gene from the halotolerant alga Dunaliella salina (2013), PLoS ONE, 8, e62287.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Dunaliella salina
gene GPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Dunaliella salina

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Dunaliella salina 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76600
-
-
 Dunaliella salina
100000
-
-
 Dunaliella salina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydroxyacetone phosphate + NADH + H+ Dunaliella salina
-
 sn-glycerol 3-phosphate + NAD+
-
 r
dihydroxyacetone phosphate + NADH + H+ Dunaliella salina 435
-
 sn-glycerol 3-phosphate + NAD+
-
 r
sn-glycerol 3-phosphate + NAD+ Dunaliella salina
-
 glycerone phosphate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Dunaliella salina Q52ZA0
-
-
Dunaliella salina V9MH41
-
-
Dunaliella salina 435 Q52ZA0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + NADH + H+
-
 Dunaliella salina sn-glycerol 3-phosphate + NAD+
-
 r
dihydroxyacetone phosphate + NADH + H+
-
 Dunaliella salina 435 sn-glycerol 3-phosphate + NAD+
-
 r
sn-glycerol 3-phosphate + NAD+
-
 Dunaliella salina glycerone phosphate + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 100000, recombinant enzyme, SDS-PAGE Dunaliella salina
? x * 76600, about, sequence calculation, x* 100000, recombinant His-tagged enzyme, SDS-PAGE Dunaliella salina
? x * 76600, calculated from amino acid sequence Dunaliella salina

Synonyms

Synonyms Comment Organism
G3PDH
-
 Dunaliella salina
NAD+-dependent glycerol-3-phosphate dehydrogenase
-
 Dunaliella salina

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Dunaliella salina
NADH
-
 Dunaliella salina

pI Value

Organism Comment pI Value Maximum pI Value
Dunaliella salina calculated from amino acid sequence
-
 6.5

General Information

General Information Comment Organism
metabolism glycerol-3-phosphate dehydrogenase (G3PDH) is a key enzyme in the pathway of glycerol synthesis, which converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate Dunaliella salina
physiological function the glycerol-DHAP cycle pathway, which is driven by enzyme G3PDH, is considered as the rate-limiting enzyme to regulate the glycerol level under osmotic shocks Dunaliella salina