General Stability | Organism |
---|---|
dithiothreitol stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-diphospho-D-glycerate | 1 mM, 83% and 91% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively | Bos taurus | |
2-phospho-DL-glycerate | 1 mM, 93% and 81% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively | Bos taurus | |
3-phospho-D-glycerate | 1 mM, 53% and 65% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively | Bos taurus | |
Ag+ | 0.1 mM, complete inhibition | Bos taurus | |
alpha-D-fructose 1,6-diphosphate | 0.1 mM, 28% and 74% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively | Bos taurus | |
ATP | 1 mM, 62% and 64% inhibition of liver and spinal cord enzyme respectively, 73% and 89% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively | Bos taurus | |
Hg2+ | 0.1 mM, complete inhibition | Bos taurus | |
p-chloromercuribenzoate | 0.1 mM, complete inhibition | Bos taurus | |
pyruvate | 1 mM, 73% and 89% inhibition of hydroxypyruvate reduction and D-glycerate oxidation respectively | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Hydroxypyruvate | - |
Bos taurus | |
0.037 | - |
NADP+ | - |
Bos taurus | |
0.077 | - |
NAD+ | - |
Bos taurus | |
1.4 | - |
DL-glycerate | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
sedimentation velocity analysis | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NAD(P)H | Bos taurus | - |
D-glycerate + NAD(P)+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.67 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-glycerate + NAD+ | - |
Bos taurus | 3-hydroxypyruvate + NADH + H+ | - |
r | |
hydroxypyruvate + NAD(P)H | - |
Bos taurus | D-glycerate + NAD(P)+ + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
hydroxypyruvate reduction | Bos taurus |
9.3 | - |
D-glycerate oxidation | Bos taurus |