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Literature summary for 1.1.1.83 extracted from
- Purification and characterization of a bifunctional L-(+)-tartrate dehydrogenase-D-(+)-malate dehydrogenase (decarboxylating) from Rhodopseudomonas sphaeroides Y (1983), J. Bacteriol., 155, 281-290.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | optimal activity in presence of both Mn2+ and NH4+ | Cereibacter sphaeroides |  |
General Stability
| General Stability | Organism |
|---|---|
| 10-40% loss of activity during repeated freezing and thawing | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Cereibacter sphaeroides | |
| Dihydroxyfumarate | noncompetitive | Cereibacter sphaeroides | |
| iodoacetate | - |
Cereibacter sphaeroides | |
| L-Tartrate | competitive with respect to D-malate | Cereibacter sphaeroides | |
| meso-tartrate | competitive | Cereibacter sphaeroides | |
| oxaloacetate | competitive | Cereibacter sphaeroides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
NAD+ | - |
Cereibacter sphaeroides | |
| 0.17 | - |
D-malate | - |
Cereibacter sphaeroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, 30% of the activity with Mn2+ | Cereibacter sphaeroides | |
| K2SO4 | 50 mM, 56% of the activity with Mn2+ | Cereibacter sphaeroides | |
| KCl | 50 mM, 63% of the activity with Mn2+ | Cereibacter sphaeroides | |
| Mg2+ | 5 mM, 59% of the activity with Mn2+ | Cereibacter sphaeroides | |
| Mn2+ | Km: 0.016 mM, optimal activity in presence of both Mn2+ and NH4+ | Cereibacter sphaeroides | |
| NaCl | 50 mM, 19% of the activity with Mn2+ | Cereibacter sphaeroides | |
| Zn2+ | 0.1 mM, 14% of the activity with Mn2+ | Cereibacter sphaeroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38500 | - |
4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
| 158000 | - |
gel filtration | Cereibacter sphaeroides |
| 162000 | - |
equilibrium sedimentation | Cereibacter sphaeroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
| Cereibacter sphaeroides Y | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bifunctional L-(+)-tartrate-D-(+)-malate dehydrogenase | Cereibacter sphaeroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.6 | - |
- |
Cereibacter sphaeroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for months | Cereibacter sphaeroides |
| 0°C, stable for 30 h, even at protein concentration below 1 mg/ml | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-malate + NAD+ | - |
Cereibacter sphaeroides | pyruvate + CO2 + NADH | - |
? | |
| (R)-malate + NAD+ | - |
Cereibacter sphaeroides Y | pyruvate + CO2 + NADH | - |
? | |
| additional information | bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate | Cereibacter sphaeroides | ? | - |
? | |
| additional information | bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate | Cereibacter sphaeroides Y | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cereibacter sphaeroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: 30 h | Cereibacter sphaeroides |
| 50 | - |
half-life: 120 min | Cereibacter sphaeroides |
| 55 | - |
half-life: 30 min | Cereibacter sphaeroides |
| 60 | - |
half-life: 9 min | Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | 9 | - |
Cereibacter sphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10.6 | at pH 7.0 and 10.6: about 50% of activity maximum | Cereibacter sphaeroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
30°C, 4 h, stable | Cereibacter sphaeroides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Cereibacter sphaeroides | |
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