Literature summary for 1.1.1.85 extracted from

Urano, N.; Kanayama, H.; Karube, I.
Restriction mapping and partial characterization of beta-isopropylmalate dehydrogenase gene cloned from Citrobacter freundii (1985), J. Biotechnol., 2, 39-45.

No PubMed abstract available

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Application

Application	Comment	Organism
analysis	the cloned 3-isopropylmalate dehydrogenase gene can be used as a genetic marker in constructing vectors in Citrobacter freundii	Citrobacter freundii

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Bacillus subtilis using pLS353 as a vector and PstI as an enzyme, enzyme activities in both Escherichia coli and Bacillus subtilis transformants are 4-8fold higher than those in the present strains	Citrobacter freundii

Organism

Organism	UniProt	Comment	Textmining
Citrobacter freundii	-	TIT 01010	-
Citrobacter freundii TIT 01010	-	TIT 01010	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2R,3S)-3-isopropylmalate + NAD+	-	Citrobacter freundii	2-oxoisocaproate + NADH + H+ + CO2	-	?
(2R,3S)-3-isopropylmalate + NAD+	-	Citrobacter freundii TIT 01010	2-oxoisocaproate + NADH + H+ + CO2	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Citrobacter freundii

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Release 2023.1 (January 2023)