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Literature summary for 1.1.1.85 extracted from
- 3-Isopropylmalate dehydrogenase from chemolithoautotroph Thiobacillus ferrooxidans: DNA sequence, enzyme purification, and characterization (1993), J. Biochem., 114, 370-377.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans |  |
| iodoacetate | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans | |
| NEM | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans | |
| PCMB | 5 mM, 50% inhibition | Acidithiobacillus ferrooxidans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.026 | - |
(2R,3S)-3-isopropylmalate | pH 9.0 | Acidithiobacillus ferrooxidans | |
| 0.8 | - |
NAD+ | pH 9.0 | Acidithiobacillus ferrooxidans | |
| 4 | - |
D-malate | pH 9.0 | Acidithiobacillus ferrooxidans | |
| 12 | - |
L-malate | pH 9.0 | Acidithiobacillus ferrooxidans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | monovalent cation required | Acidithiobacillus ferrooxidans | |
| Mg2+ | 1.0 mM, enhances activity 4.7fold | Acidithiobacillus ferrooxidans | |
| Mn2+ | 1.0 mM, enhances activity 4.2fold | Acidithiobacillus ferrooxidans | |
| NH4+ | monovalent cation required | Acidithiobacillus ferrooxidans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Acidithiobacillus ferrooxidans |
| 58000 | - |
gel filtration | Acidithiobacillus ferrooxidans |
| 73000 | - |
equilibrium sedimentation | Acidithiobacillus ferrooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme expressed in Escherichia coli | Acidithiobacillus ferrooxidans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 138.2 | - |
- |
Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-3-isopropylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
| (2R,3S)-3-isopropylmalate + NAD+ | - |
Acidithiobacillus ferrooxidans | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | 12% of the activity with 3-isopropylmalate | Acidithiobacillus ferrooxidans | pyruvate + NADH + CO2 | - |
? | |
| D-malate + NAD+ | 19% of the activity with 3-isopropylmalate | Acidithiobacillus ferrooxidans | pyruvate + NADH + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Acidithiobacillus ferrooxidans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acidithiobacillus ferrooxidans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 40% loss of activity | Acidithiobacillus ferrooxidans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Acidithiobacillus ferrooxidans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Acidithiobacillus ferrooxidans | |
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