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Literature summary for 1.1.1.85 extracted from

  • Wallon, G.; Yamamoto, K.; Kirino, H.; Yamagishi, A.; Lovett, S.T.; Petsko, G.A.; Oshima, T.
    Purification, catalytic properties and thermostability of 3-isopropylmalate dehydrogenase from Escherichia coli (1997), Biochim. Biophys. Acta, 1337, 105-112.
    View publication on PubMed
Search for more literature for 1.1.1.85

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 isopropylmalate pH 7.6, 60°C Thermus thermophilus
0.09
-
 ethylmalate pH 7.6 Escherichia coli
0.105
-
 propylmalate pH 7.6 Escherichia coli
0.105
-
 isopropylmalate pH 7.6, 40°C Escherichia coli
0.139
-
 NAD+ pH 7.6, 60°C Thermus thermophilus
0.157
-
 NAD+ pH 7.6, 40°C Thermus thermophilus
0.214
-
 tert-butylmalate pH 7.6 Escherichia coli
0.316
-
 isopropylmalate pH 7.6, 40°C Thermus thermophilus
0.321
-
 NAD+ pH 7.6, 40°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Thermus thermophilus
K+ 0.3 mM required for optimal activity Escherichia coli
Mg2+
-
 Thermus thermophilus
Mg2+ divalent cation required, saturation is reached at 0.2 mM, activity with Mn2+ is twice as high as with Mg2+ Escherichia coli
Mn2+
-
 Thermus thermophilus
Mn2+ divalent cation required, saturation is reached at 0.2 mM, activity with Mn2+ is twice as high as with Mg2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermus thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Escherichia coli Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
(2R,3S)-3-isopropylmalate + NAD+
-
 Escherichia coli 2-oxoisocaproate + NADH + H+ + CO2
-
 ?
ethylmalate + NAD+
-
 Escherichia coli 2-oxopentanoate + NADH + H+ + CO2
-
 ?
isopropylmalate + NAD+
-
 Escherichia coli ?
-
 ?
propylmalate + NAD+
-
 Escherichia coli ?
-
 r
tert-butylmalate + NAD+
-
 Escherichia coli 2-hydroxy-4,4-dimethylpentanoate + NADH + H+ + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Escherichia coli
80
-
-
 Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 45% of maximal activity, 80°C: about 85% of maximal activity Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
63
-
 midpoint of irreversible denaturation Escherichia coli
64
-
-
 Thermus thermophilus
64
-
 10 min, 50% inactivation Escherichia coli
79
-
 midpoint of irreversible denaturation Thermus thermophilus
83
-
 10 min, 50% inactivation Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 tert-butylmalate pH 7.6 Escherichia coli
8
-
 isopropylmalate pH 7.6, 70°C Thermus thermophilus
8
-
 NAD+ pH 7.6, 70°C, reaction with isopropylmalate Thermus thermophilus
19
-
 isopropylmalate pH 7.6, 40°C Thermus thermophilus
19
-
 NAD+ pH 7.6, 40°C, reaction with isopropylmalate Thermus thermophilus
69
-
 isopropylmalate pH 7.6, 40°C Escherichia coli
69
-
 NAD+ pH 7.6, 40°C, reaction with isopropylmalate Escherichia coli
70
-
 ethylmalate pH 7.6 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermus thermophilus
NAD+
-
 Escherichia coli