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Literature summary for 1.1.1.86 extracted from

  • Kiritani, K.; Narise, S.; Wagner, R.P.
    The reductoisomerase of Neurospora crassa (1966), J. Biol. Chem., 241, 2047-2051.
No PubMed abstract available

General Stability

General Stability Organism
unstable in absence of NADPH and Mg2+, very unstable in Tris buffer, 2-mercaptoethanol stabilizes Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
NADH
-
Neurospora crassa
NADP+
-
Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
2-aceto-2-hydroxybutyrate pH 7.5, 25°C Neurospora crassa
0.32
-
2-acetolactate pH 7.5, 25°C Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Neurospora crassa
Mg2+ optimum concentration 5 mM Neurospora crassa
additional information metal ions other than Mg2+ are inhibitory Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-acetolactate + NADPH Neurospora crassa enzyme of branched chain amino acid synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH Neurospora crassa enzyme of valine synthesis 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
NADPH + alpha-aceto-alpha-hydroxybutyrate Neurospora crassa enzyme of isoleucine biosynthesis NADP+ + ?
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
lipoprotein purified enzyme contains 39-46% lipid Neurospora crassa

Purification (Commentary)

Purification (Comment) Organism
-
Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.5
-
-
Neurospora crassa

Storage Stability

Storage Stability Organism
-10°, in presence of NADPH, 5 d, 70-80% loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetolactate + NADPH enzyme of branched chain amino acid synthesis Neurospora crassa 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
2-acetolactate + NADPH enzyme of valine synthesis Neurospora crassa 2,3-dihydroxy-3-methylbutanoate + NADP+
-
r
NADPH + 2-aceto-2-hydroxybutyrate activity is 6-10times greater than that for 2-acetolactate Neurospora crassa NADP+ + ?
-
?
NADPH + 2-acetolactate
-
Neurospora crassa NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate
-
?
NADPH + alpha-aceto-alpha-hydroxybutyrate enzyme of isoleucine biosynthesis Neurospora crassa NADP+ + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, about 90% loss of activity at pH 6.2, about 55% loss of activity at pH 6.5, 35% loss of activity oH 7.0, 30% loss of activity at pH 7.5, about 85% loss of activity at pH 8.0, about 90% loss of activity at pH 8.5 Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
2-acetolactate as substrate Neurospora crassa
7.5
-
alpha-aceto-alpha-hydroxybutyrate Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.8 pH 6.6: about 65% of maximal activity, pH 8.8: about 90% of maximal activity, reaction with 2-acetolactate, Tris buffer Neurospora crassa
7 8.5 pH 7.0: about 60% of maximal activity, pH 8.5: about 65% of maximal activity Neurospora crassa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.2
-
40°C, 5 min, 90% loss of activity Neurospora crassa
6.5
-
40°C, 5 min, 55% loss of activity Neurospora crassa
7
-
40°C, 5 min, 35% loss of activity Neurospora crassa
7.5
-
40°C, 5 min, 30% loss of activity Neurospora crassa
8
-
40°C, 5 min, 85% loss of activity Neurospora crassa
8.5
-
40°C, 5 min, 90% loss of activity Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Neurospora crassa