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Literature summary for 1.1.1.88 extracted from

  • Peacock, R.B.; Hicks, C.W.; Walker, A.M.; Dewing, S.M.; Lewis, K.M.; Abboud, J.C.; Stewart, S.W.A.; Kang, C.; Watson, J.M.
    Structural and functional characterization of dynamic oligomerization in Burkholderia cenocepacia HMG-CoA reductase (2019), Biochemistry, 58, 3960-3970 .
    View publication on PubMed
Search for more literature for 1.1.1.88

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in the hexameric state bound to coenzyme A and ADP, hanging drop vapor diffusion method, using 0.2 M potassium acetate (pH 7.5) and 20% (w/v) PEG 3350 Burkholderia cenocepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-mevalonate + CoA + 2 NAD+ Burkholderia cenocepacia
-
 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 ?
(R)-mevalonate + CoA + 2 NAD+ Burkholderia cenocepacia 32315
-
 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia cenocepacia B4EKH5
-
-
Burkholderia cenocepacia 32315 B4EKH5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.89
-
 at pH 9.0 and 37°C Burkholderia cenocepacia
6
-
 at pH 7.5 and 37°C Burkholderia cenocepacia
11
-
 at pH 8.3 and 37°C Burkholderia cenocepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mevalonate + CoA + 2 NAD+
-
 Burkholderia cenocepacia 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 ?
(R)-mevalonate + CoA + 2 NAD+
-
 Burkholderia cenocepacia 32315 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Burkholderia cenocepacia

Synonyms

Synonyms Comment Organism
HMG-CoA reductase
-
 Burkholderia cenocepacia
HMGR
-
 Burkholderia cenocepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Burkholderia cenocepacia

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Burkholderia cenocepacia