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Literature summary for 1.1.1.9 extracted from

  • Jakoby, W.B.; Fredericks, J.
    Erythritol dehydrogenase from Aerobacter aerogenes (1961), Biochim. Biophys. Acta, 48, 26-32.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Klebsiella aerogenes
EDTA stabilizes during purification Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Klebsiella aerogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Klebsiella aerogenes 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation at high concentration Klebsiella aerogenes
Mn2+ requirement Klebsiella aerogenes
additional information no activation by Zn2+, Cu2+, Ca2+, Ni2+, Co2+, Fe2+ Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
2°C, 90% of maximal activity retained at pH 6.8 and 7.4 after 24 h Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD+
-
Klebsiella aerogenes ?
-
?
D-xylulose + NADH + H+
-
Klebsiella aerogenes xylitol + NAD+
-
r
dihydroxyacetone + NADH
-
Klebsiella aerogenes glycerol + NAD+
-
?
erythritol + NAD+
-
Klebsiella aerogenes L-erythrulose + NADH
-
r
glycerol + NAD+
-
Klebsiella aerogenes ?
-
?
L-arabitol + NAD+
-
Klebsiella aerogenes ?
-
?
L-erythrulose + NADH
-
Klebsiella aerogenes erythritol + NAD+
-
r
L-xylulose + NADH
-
Klebsiella aerogenes L-xylitol + NAD+
-
?
additional information no oxidation of threitol, xylitol, sorbitol, D-iditol Klebsiella aerogenes ?
-
?
additional information no reduction of D-fructose, L-sorbose and D-tagatose Klebsiella aerogenes ?
-
?
additional information specific for polyols of 5 or less carbon bearing cis-hydroxy groups in C2 and C3 Klebsiella aerogenes ?
-
?
xylitol + NAD+
-
Klebsiella aerogenes D-xylulose + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
reduction of erythrulose Klebsiella aerogenes
8.6
-
oxidation of erythritol Klebsiella aerogenes

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8 7.4 90% of maximal activity retained at pH 6.8 and 7.4 at 2°C Klebsiella aerogenes
7
-
24 h stable at 2°C Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Klebsiella aerogenes
additional information no activity with NADP+ Klebsiella aerogenes
additional information at high concentrations of xylitol 1.5% as effective as NAD+ Klebsiella aerogenes
NAD+
-
Klebsiella aerogenes
NADH
-
Klebsiella aerogenes