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Literature summary for 1.1.1.90 extracted from

  • Chalmers, R.M.; Scott, A.J.; Fewson, C.A.
    Purification of the benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase encoded by the TOL plasmid pWW53 of Pseudomonas putida MT53 and their preliminary comparison with benzyl alcohol dehydrogenase and benzaldehyde dehydrogenases I and II from Acinetobacter calcoaceticus (1990), J. Gen. Microbiol., 136, 637-643.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0406
-
NAD+ chromosome-encoded enzyme Pseudomonas putida
0.081
-
3-methylbenzyl alcohol plasmid-encoded enzyme Pseudomonas putida
0.106
-
4-methylbenzyl alcohol plasmid-encoded enzyme Pseudomonas putida
0.118
-
4-methylbenzyl alcohol chromosome-encoded enzyme Pseudomonas putida
0.121
-
benzyl alcohol chromosome-encoded enzyme Pseudomonas putida
0.146
-
3-methylbenzyl alcohol chromosome-encoded enzyme Pseudomonas putida
0.219
-
NAD+ plasmid-encoded enzyme Pseudomonas putida
0.233
-
benzyl alcohol plasmid-encoded enzyme Pseudomonas putida
0.605
-
2-Methylbenzyl alcohol plasmid-encoded enzyme Pseudomonas putida
0.992
-
2-Methylbenzyl alcohol chromosome-encoded enzyme Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
4 * 43000, SDS-PAGE Pseudomonas putida
82000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzyl alcohol + NAD+ Pseudomonas putida
-
benzaldehyde + NADH
-
?
benzyl alcohol + NAD+ Acinetobacter calcoaceticus
-
benzaldehyde + NADH
-
?
benzyl alcohol + NAD+ Pseudomonas putida MT53
-
benzaldehyde + NADH
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme encoded by plasmid TOL Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
376
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
-20Ā°C, concentrated, mixed with ethanediol Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbenzyl alcohol + NAD+
-
Pseudomonas putida 2-methylbenzaldehyde + NADH + H+
-
?
2-methylbenzyl alcohol + NAD+
-
Pseudomonas putida MT53 2-methylbenzaldehyde + NADH + H+
-
?
3-methylbenzyl alcohol + NAD+
-
Pseudomonas putida 3-methylbenzaldehyde + NADH + H+
-
?
3-methylbenzyl alcohol + NAD+
-
Pseudomonas putida MT53 3-methylbenzaldehyde + NADH + H+
-
?
4-methylbenzyl alcohol + NAD+
-
Pseudomonas putida 4-methylbenzaldehyde + NADH + H+
-
?
4-methylbenzyl alcohol + NAD+
-
Pseudomonas putida MT53 4-methylbenzaldehyde + NADH + H+
-
?
benzyl alcohol + NAD+
-
Pseudomonas putida benzaldehyde + NADH
-
?
benzyl alcohol + NAD+
-
Acinetobacter calcoaceticus benzaldehyde + NADH
-
?
benzyl alcohol + NAD+
-
Pseudomonas putida MT53 benzaldehyde + NADH
-
?

Subunits

Subunits Comment Organism
tetramer
-
Acinetobacter calcoaceticus
tetramer 4 * 43000, SDS-PAGE Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Acinetobacter calcoaceticus
NAD+ specific for Pseudomonas putida
NADH
-
Pseudomonas putida
NADH
-
Acinetobacter calcoaceticus