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Literature summary for 1.1.1.90 extracted from

  • Shaw, J.P.; Harayama, S.
    Purification and characterisation of TOL plasmid-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase of Pseudomonas putida (1990), Eur. J. Biochem., 191, 705-714.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Pseudomonas putida
benzyl alcohol stabilizes slightly Pseudomonas putida
dithiothreitol stabilizes Pseudomonas putida
MgCl2 stabilizes slightly Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol above 2.5 mM Pseudomonas putida
iodoacetamide slight Pseudomonas putida
N-ethylmaleimide
-
Pseudomonas putida
NADH noncompetitive Pseudomonas putida
p-chloromercuribenzoate
-
Pseudomonas putida
SH-blocking agents
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0257
-
benzaldehyde
-
Pseudomonas putida
0.047
-
NADH
-
Pseudomonas putida
0.0476
-
NAD+
-
Pseudomonas putida
0.2194
-
benzyl alcohol
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
KBr
-
Pseudomonas putida
KCl activity nearly doubled Pseudomonas putida
KNO3
-
Pseudomonas putida
MgCl2 slight activation Pseudomonas putida
NaCl
-
Pseudomonas putida
NH4Cl
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Pseudomonas putida
90000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzyl alcohol + NAD+ Pseudomonas putida
-
benzaldehyde + NADH
-
r
benzyl alcohol + NAD+ Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
benzaldehyde + NADH
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme encoded by plasmid TOL Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
230.3
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
-70┬░C, enzyme dialyzed against buffer containing 40% glycerol Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzyl alcohol + NAD+
-
Pseudomonas putida 2-aminobenzaldehyde + NADH
-
r
2-hydroxymethylthiophene + NAD+
-
Pseudomonas putida 2-formylthiophene + NADH
-
r
2-methylbenzyl alcohol + NAD+
-
Pseudomonas putida 2-methylbenzaldehyde + NADH + H+
-
r
2-methylbenzyl alcohol + NAD+
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-methylbenzaldehyde + NADH + H+
-
r
3,4-dimethylbenzyl alcohol + NAD+
-
Pseudomonas putida 3,4-dimethylbenzaldehyde + NADH + H+
-
r
3-aminobenzyl alcohol + NAD+
-
Pseudomonas putida 3-aminobenzaldehyde + NADH
-
r
3-chlorobenzyl alcohol + NAD+
-
Pseudomonas putida 3-chlorobenzaldehyde + NADH
-
r
3-hydroxybenzyl alcohol + NAD+
-
Pseudomonas putida 3-hydroxybenzaldehyde + NADH
-
r
3-methoxybenzyl alcohol + NAD+
-
Pseudomonas putida 3-methoxybenzaldehyde + NADH
-
r
3-methylbenzyl alcohol + NAD+
-
Pseudomonas putida 3-methylbenzaldehyde + NADH + H+
-
r
3-nitrobenzyl alcohol + NAD+
-
Pseudomonas putida 3-nitrobenzaldehyde + NADH
-
r
4-aminobenzyl alcohol + NAD+
-
Pseudomonas putida 4-aminobenzaldehyde + NADH
-
r
4-methylbenzyl alcohol + NAD+
-
Pseudomonas putida 4-methylbenzaldehyde + NADH + H+
-
r
benzyl alcohol + NAD+
-
Pseudomonas putida benzaldehyde + NADH
-
r
benzyl alcohol + NAD+
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 benzaldehyde + NADH
-
r
furfuryl alcohol + NAD+
-
Pseudomonas putida furfural + NADH
-
r
furfuryl alcohol + NAD+
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 furfural + NADH
-
r
additional information not: 4-nitrophenyl acetate Pseudomonas putida ?
-
?
additional information a group of enzymes with broad specificity towards alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short chain aliphatic alcohols Pseudomonas putida ?
-
?
additional information not: 4-nitrophenyl acetate Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
?
additional information a group of enzymes with broad specificity towards alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short chain aliphatic alcohols Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Pseudomonas putida

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
37
-
after 21 h no loss of activity Pseudomonas putida
56
-
after 10 min complete loss of activity Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
228
-
benzyl alcohol
-
Pseudomonas putida
622
-
benzaldehyde
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10.5 pH 7.5: about 15% of activity maximum, pH 10.5: about 20% of activity maximum Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
additional information contains no prosthetic group such as FAD or FMN Pseudomonas putida
NAD+ enzyme transfers hydride to the pro-R side of the prochiral C4 to the pyridine ring of NAD+ Pseudomonas putida
NADH
-
Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.8
-
NADH
-
Pseudomonas putida