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Literature summary for 1.1.1.95 extracted from
- Phosphoglycerate dehydrogenase from soybean nodules (1983), Plant Physiol., 71, 658-661.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| HPO42- | - |
Glycine max |  |
| SO42- | - |
Glycine max | |
General Stability
| General Stability | Organism |
|---|---|
| dilution inactivates | Glycine max |
| dithiothreitol increases stability | Glycine max |
| inorganic ions, i.e. Cl-, SO42-, HPO42-, essential for stability | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | noncompetitive to phosphohydroxypyruvate | Glycine max | |
| L-serine | not | Glycine max | |
| NAD+ | product inhibition, competitive to NADH | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
NADH | pH 7.5 | Glycine max | |
| 0.15 | - |
NADPH | pH 7.5 | Glycine max | |
| 0.15 | - |
D-3-phosphoglycerate | pH 7.5 | Glycine max | |
| 0.25 | - |
NAD+ | pH 9.4 | Glycine max | |
| 0.29 | - |
D-3-phosphoglycerate | pH 9.4 | Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | stimulates | Glycine max | |
| MgCl2 | stimulates | Glycine max | |
| SO42- | stimulates | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | - |
gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phosphoglycerate + NAD+ | Glycine max | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | 3-phosphohydroxypyruvate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| nodule | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.3 | - |
- |
Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NADP+ | - |
Glycine max | 3-phosphohydroxypyruvate + NADPH + H+ | - |
? | |
| 3-phosphoglycerate + NAD+ | - |
Glycine max | 3-phosphohydroxypyruvate + NADH | - |
r | |
| 3-phosphoglycerate + NAD+ | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | Glycine max | 3-phosphohydroxypyruvate + NADH | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
NADH oxidation | Glycine max |
| 9.4 | - |
NAD+ reduction | Glycine max |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.8 | about 50% of activity maximum at pH 5 and 7.8, NADH oxidation | Glycine max |
| 8.5 | 10.3 | about 50% of activity maximum at pH 8.5 and 10.3, NAD+ reduction | Glycine max |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Glycine max | |
| NADH | - |
Glycine max | |
| NADPH | - |
Glycine max | |
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Release 2023.1 (January 2023)
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