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Literature summary for 1.1.1.95 extracted from

  • Shimizu, Y.; Sakuraba, H.; Doi, K.; Ohshima, T.
    Molecular and functional characterization of D-3-phosphoglycerate dehydrogenase in the serine biosynthetic pathway of the hyperthermophilic archaeon Sulfolobus tokodaii (2008), Arch. Biochem. Biophys., 470, 120-128.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 enhancing effect on NAD-dependent 3-phospho-D-glycerate oxidation and 3-phosphohydroxypyruvate reduction but inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction Sulfurisphaera tokodaii
K2HPO4 enhancing effect on NAD-dependent 3-phospho-D-glycerate oxidation and 3-phosphohydroxypyruvate reduction but inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction Sulfurisphaera tokodaii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Sulfurisphaera tokodaii

General Stability

General Stability Organism
enzyme is highly thermostable Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction Sulfurisphaera tokodaii
3-phosphohydroxypyruvate product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: 3-phospho-D-glycerate, inhibition pattern: competitive; product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: NAD, inhibition pattern: non-competitive Sulfurisphaera tokodaii
ADP weak inhibitory effect on 3-phospho-D-glycerate oxidation, remaining activity: 86% Sulfurisphaera tokodaii
AMP weak inhibitory effect on 3-phospho-D-glycerate oxidation, remaining activity: 75% Sulfurisphaera tokodaii
ATP weak inhibitory effect on 3-phospho-D-glycerate oxidation, remaining activity: 85% Sulfurisphaera tokodaii
K2HPO4 inhibitory effect on NADPH-dependent 3-phosphohydroxypyruvate reduction. It appears that the phosphate ion PO43- exerts its inhibitory effect by binding to the free enzyme and NADPH-enzyme complex Sulfurisphaera tokodaii
additional information unlike the Escherichia coli PGDH no inhibition by L-serine Sulfurisphaera tokodaii
NADH product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: 3-phospho-D-glycerate, inhibition pattern: non-competitive; product inhibition of 3-phospho-D-glycerate oxidation, variable substrate: NAD, inhibition pattern: competitive Sulfurisphaera tokodaii
ZnCl2 inhibitory effect on 3-phosphohydroxypyruvate reduction, remaining activity: 29% Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
NADH value for 3-phosphohydroxypyruvate reduction using NADH as a cofactor Sulfurisphaera tokodaii
0.0096
-
3-phosphohydroxypyruvate value for 3-phosphohydroxypyruvate reduction using NADH as a cofactor Sulfurisphaera tokodaii
0.02
-
NADPH value for 3-phosphohydroxypyruvate reduction using NADPH as a cofactor Sulfurisphaera tokodaii
0.033
-
3-phospho-D-glycerate value for 3-phospho-D-glycerate oxidation using NAD+ as a cofactor Sulfurisphaera tokodaii
0.04
-
NAD+ value for 3-phospho-D-glycerate oxidation Sulfurisphaera tokodaii
0.12
-
3-phosphohydroxypyruvate value for 3-phosphohydroxypyruvate reduction using NADPH as a cofactor Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a butyl Sepharose 4 Fast Flow column and a Superdex 200 gel filtration column. During purification an unexpected decline in enzyme activity is observed if the enzyme is stored in plastic tubes, but not in glass ones Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
during purification an unexpected decline in enzyme activity is observed if the enzyme is stored in plastic tubes, but not in glass ones Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate + NAD+ activity relative to 3-phospho-D-glycerate: 47% Sulfurisphaera tokodaii 2-phosphohydroxypyruvate + NADH + H+
-
?
3-phospho-D-glycerate + NAD+ highest activity Sulfurisphaera tokodaii 3-phosphohydroxypyruvate + NADH + H+
-
r
3-phospho-D-glycerate + NADP+
-
Sulfurisphaera tokodaii 3-phosphohydroxypyruvate + NADPH + H+
-
r
DL-glyceraldehyde 3-phosphate + NAD+ activity relative to 3-phospho-D-glycerate: 9% Sulfurisphaera tokodaii ?
-
?

Subunits

Subunits Comment Organism
homodimer gel filtration Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
D-3-phosphoglycerate dehydrogenase
-
Sulfurisphaera tokodaii
PGDH
-
Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Sulfurisphaera tokodaii
53
-
optimum temperature for 3-phospho-D-glycerate oxidation Sulfurisphaera tokodaii
80
-
optimum for 3-phosphohydroxypyruvate reduction Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 enzyme is highly thermostable, retaining more than 90% of its activity after incubation for 1 h at 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
3-phosphohydroxypyruvate reduction Sulfurisphaera tokodaii
10
-
3-phospho-D-glycerate oxidation Sulfurisphaera tokodaii

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 11.5 enzyme is stable over a wide range of pHs. No decrease in activity after 20 min incubation at 50°C Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Sulfurisphaera tokodaii
NADP+ NADP utilization is not observed in the forward reaction even in the presence of high concentrations of 10 mM NADP and 10 mM 3-phospho-D-glycerate Sulfurisphaera tokodaii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
K2HPO4 value of phosphate against 3-phosphohydroxypyruvate Sulfurisphaera tokodaii
10
-
K2HPO4 value of phosphate against NADPH Sulfurisphaera tokodaii