Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Entamoeba histolytica |
Crystallization (Comment) | Organism |
---|---|
in complexes with 3-phospho-D-glycerate and NAD+, hanging drop vapor diffusion method, using 20% (w/v) poly(ethylene glycol) 3350 in 100 mM Tris pH 7.0-8.5, 350 mM sodium formate and 5% (v/v) glycerol | Entamoeba histolytica |
Protein Variants | Comment | Organism |
---|---|---|
K263A | the mutant shows strongly reduced activity compared to the wild type enzyme | Entamoeba histolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.396 | - |
3-phospho-D-glycerate | wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica | |
0.6 | - |
3-phospho-D-glycerate | mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | Entamoeba histolytica | - |
3-phosphonooxypyruvate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q76KF5 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-Sepharose column chromatography and Superdex 200 gel filtration | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica |
0.495 | - |
wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Entamoeba histolytica | 3-phosphonooxypyruvate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
PGDH | - |
Entamoeba histolytica |
type III 3-phosphoglycerate dehydrogenase | - |
Entamoeba histolytica |
type IIIK PGDH | - |
Entamoeba histolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
3-phospho-D-glycerate | mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica | |
16.28 | - |
3-phospho-D-glycerate | wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Entamoeba histolytica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.216 | - |
3-phospho-D-glycerate | mutant enzyme K263A, at pH 9.0 and 25°C | Entamoeba histolytica | |
4.2 | - |
3-phospho-D-glycerate | wild type enzyme, at pH 9.0 and 25°C | Entamoeba histolytica |