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Literature summary for 1.1.1.95 extracted from
- Determinants of substrate specificity in D-3-phosphoglycerate dehydrogenase. Conversion of the M. tuberculosis enzyme from one that does not use alpha-ketoglutarate as a substrate to one that does (2019), Arch. Biochem. Biophys., 671, 218-224 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K141R | kcat/KM for 2-oxoglutarate is 4.8fold lower than the value for the wild-type enzyme, kcat/KM for 3-phosphooxypyruvate is 2.6fold higher than the value for the wild-type enzyme | Escherichia coli |
| R132K | mutation decreases the Km-value for 3-phosphooxypyruvate by approximately 10fold | Mycobacterium tuberculosis |
| R72A | mutant enzyme utilize 2-oxoglutarate as substrate | Mycobacterium tuberculosis |
| R72L | mutant enzyme utilize 2-oxoglutarate as substrate | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
3-phosphooxypyruvate | mutant enzyme R132K, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis |  |
| 0.068 | - |
3-phosphooxypyruvate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 0.081 | - |
3-phosphooxypyruvate | mutant enzyme K141R, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 0.153 | - |
3-phosphooxypyruvate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.169 | - |
3-phosphooxypyruvate | mutant enzyme R72A/R132K, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.45 | - |
2-oxoglutarate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 5 | - |
2-oxoglutarate | mutant enzyme K141R, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 30.6 | - |
2-oxoglutarate | mutant enzyme R72L, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 53.1 | - |
2-oxoglutarate | mutant enzyme R72A, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phospho-D-glycerate + NAD+ | Mycobacterium tuberculosis | first step of L-serine biosynthesis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | Escherichia coli | first step of L-serine biosynthesis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | Mycobacterium tuberculosis H37Rv | first step of L-serine biosynthesis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | Escherichia coli K12 | first step of L-serine biosynthesis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A9T0 | - |
- |
| Escherichia coli K12 | P0A9T0 | - |
- |
| Mycobacterium tuberculosis | P9WNX3 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WNX3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NADH + H+ | - |
Escherichia coli | D-2-hydroxyglutarate + NAD+ | - |
? | |
| 2-oxoglutarate + NADH + H+ | wild-type enzyme shows no activity. Mutant enzymes R72A and R72L utilize 2-oxoglutarate as substrate | Mycobacterium tuberculosis | D-2-hydroxyglutarate + NAD+ | - |
? | |
| 2-oxoglutarate + NADH + H+ | wild-type enzyme shows no activity. Mutant enzymes R72A and R72L utilize 2-oxoglutarate as substrate | Mycobacterium tuberculosis H37Rv | D-2-hydroxyglutarate + NAD+ | - |
? | |
| 2-oxoglutarate + NADH + H+ | - |
Escherichia coli K12 | D-2-hydroxyglutarate + NAD+ | - |
? | |
| 3-phospho-D-glycerate + NAD+ | - |
Mycobacterium tuberculosis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | - |
Escherichia coli | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | first step of L-serine biosynthesis | Mycobacterium tuberculosis | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | first step of L-serine biosynthesis | Escherichia coli | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | - |
Mycobacterium tuberculosis H37Rv | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | first step of L-serine biosynthesis | Mycobacterium tuberculosis H37Rv | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | - |
Escherichia coli K12 | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phospho-D-glycerate + NAD+ | first step of L-serine biosynthesis | Escherichia coli K12 | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Mycobacterium tuberculosis | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Escherichia coli | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Mycobacterium tuberculosis H37Rv | 3-phospho-D-glycerate + NAD+ | - |
r | |
| 3-phosphooxypyruvate + NADH + H+ | - |
Escherichia coli K12 | 3-phospho-D-glycerate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-3-phosphoglycerate dehydrogenase | - |
Mycobacterium tuberculosis |
| D-3-phosphoglycerate dehydrogenase | - |
Escherichia coli |
| PGDH | - |
Mycobacterium tuberculosis |
| PGDH | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
2-oxoglutarate | mutant enzyme K141R, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 2.1 | - |
2-oxoglutarate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 3.1 | - |
3-phosphooxypyruvate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 4.3 | - |
2-oxoglutarate | mutant enzyme R72L, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 9.6 | - |
3-phosphooxypyruvate | mutant enzyme K141R, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 16.1 | - |
2-oxoglutarate | mutant enzyme R72A, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 458 | - |
3-phosphooxypyruvate | mutant enzyme R132K, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 490 | - |
3-phosphooxypyruvate | mutant enzyme R72A/R132K, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 606 | - |
3-phosphooxypyruvate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mycobacterium tuberculosis | |
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Mycobacterium tuberculosis | |
| NADH | - |
Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | first step of L-serine biosynthesis | Mycobacterium tuberculosis |
| metabolism | first step of L-serine biosynthesis | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
2-oxoglutarate | mutant enzyme R72L, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.3 | - |
2-oxoglutarate | mutant enzyme R72A, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 0.98 | - |
2-oxoglutarate | mutant enzyme K141R, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 4.7 | - |
2-oxoglutarate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 46 | - |
3-phosphooxypyruvate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 120 | - |
3-phosphooxypyruvate | mutant enzyme K141R, pH 7.0, temperature not specified in the publication | Escherichia coli | |
| 2900 | - |
3-phosphooxypyruvate | mutant enzyme R72A/R132K, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 4000 | - |
3-phosphooxypyruvate | wild-type enzyme, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
| 31000 | - |
3-phosphooxypyruvate | mutant enzyme R132K, pH 7.0, temperature not specified in the publication | Mycobacterium tuberculosis | |
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