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Literature summary for 1.1.1.96 extracted from
- Purification, characterization and identification of aromatic 2-oxo acid reductase (1977), Life Sci., 20, 609-616.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,5-dihydroxybenzoic acid | 0.5 mM, 60% inhibition | Canis lupus familiaris |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
NADH | - |
Canis lupus familiaris | |
| 0.04 | - |
3,5-diiodo-4-hydroxyphenylpyruvate | - |
Canis lupus familiaris | |
| 0.14 | - |
oxaloacetate | - |
Canis lupus familiaris | |
| 0.2 | - |
Indolepyruvate | - |
Canis lupus familiaris | |
| 1.6 | - |
phenylpyruvate | - |
Canis lupus familiaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Canis lupus familiaris | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Canis lupus familiaris |
| 75000 | - |
sucrose-density gradient centrifugation | Canis lupus familiaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, hydroxyapatite, isoelectric focusing, Sephadex G-150 | Canis lupus familiaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Canis lupus familiaris | - |
| heart | - |
Canis lupus familiaris | - |
| kidney | - |
Canis lupus familiaris | - |
| liver | - |
Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1200 | - |
- |
Canis lupus familiaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM potassium phosphate, pH 7.5, 2 months, no loss of activity | Canis lupus familiaris |
| 4°C, 50 mM potassium phosphate, pH 7.5, 2 weeks, no loss of activity | Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH | - |
Canis lupus familiaris | 3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+ | - |
r | |
| indolepyruvate + NADH | - |
Canis lupus familiaris | indolelactate + NAD+ | - |
r | |
| oxaloacetate + NADH | order of activity, oxaloacetate, 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate, indolepyruvate, phenylpyruvate | Canis lupus familiaris | malate + NAD+ | - |
r | |
| phenylpyruvate + NADH | - |
Canis lupus familiaris | phenyllactate + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Canis lupus familiaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Canis lupus familiaris | |
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Release 2023.1 (January 2023)
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