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Literature summary for 1.1.1.B19 extracted from

  • Takata, G.; Poonperm, W.; Morimoto, K.; Izumori, K.
    Cloning and overexpression of the xylitol dehydrogenase gene from Bacillus pallidus and its application to L-xylulose production (2010), Biosci. Biotechnol. Biochem., 74, 1807-1813.
    View publication on PubMed
Search for more literature for 1.1.1.B19

Application

Application Comment Organism
synthesis production of L-xylulose from xylitol using a resting cell reaction leads to 35% L-xylulose within 24 h, starting from 5% xylitol as initial concentration Aeribacillus pallidus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeribacillus pallidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 NAD+ pH 10.0, 40°C Aeribacillus pallidus
0.83
-
 D-threitol pH 10.0, 40°C Aeribacillus pallidus
1.27
-
 L-xylulose pH 10.0, 40°C Aeribacillus pallidus
2.06
-
 xylitol pH 10.0, 40°C Aeribacillus pallidus
2.64
-
 D-arabitol pH 10.0, 40°C Aeribacillus pallidus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, 50.8% of the activity with Mn2+ Aeribacillus pallidus
Mg2+ 1 mM, 36.9% of the activity with Mn2+ Aeribacillus pallidus
Mn2+ required Aeribacillus pallidus
additional information no significant stimulation by Na+, Cu2+, K+, Zn2+, Co2+ Aeribacillus pallidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27333
-
 x * 27333, calculated, x * 28000, SDS-PAGE Aeribacillus pallidus
28000
-
 x * 27333, calculated, x * 28000, SDS-PAGE Aeribacillus pallidus

Organism

Organism UniProt Comment Textmining
Aeribacillus pallidus E0D583
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aeribacillus pallidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NADH + H+ 15% conversion in resting cell reaction Aeribacillus pallidus D-ribulose + NAD+
-
 ?
D-iditol + NAD+ 72% conversion in resting cell reaction Aeribacillus pallidus D-sorbose + NADH + H+
-
 ?
D-talitol + NADH + H+ 15% conversion in resting cell reaction Aeribacillus pallidus D-tagatose + NAD+
-
 ?
D-threitol + NAD+ 48% conversion in resting cell reaction Aeribacillus pallidus erythrulose + NADH + H+
-
 ?
D-xylulose + NADH + H+
-
 Aeribacillus pallidus xylitol + NAD+
-
 ?
additional information no substrate: DL-rhamnitol, galactitol, allitol, DL-fucitol, L-iditol, L-threitol, DL-sorbitol, DL-mannitol, erythritol Aeribacillus pallidus ?
-
 ?
ribitol + NADH + H+ 15% conversion in resting cell reaction Aeribacillus pallidus D-ribulose + NAD+
-
 ?
xylitol + NAD+ 50% conversion in resting cell reaction Aeribacillus pallidus L-xylulose + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 27333, calculated, x * 28000, SDS-PAGE Aeribacillus pallidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aeribacillus pallidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, 66% residual acitivity Aeribacillus pallidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.695
-
 D-arabitol pH 10.0, 40°C Aeribacillus pallidus
1.11
-
 L-xylulose pH 10.0, 40°C Aeribacillus pallidus
2.75
-
 xylitol pH 10.0, 40°C Aeribacillus pallidus
2.75
-
 D-threitol pH 10.0, 40°C Aeribacillus pallidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Aeribacillus pallidus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ Aeribacillus pallidus
NAD+ specific for NAD+ Aeribacillus pallidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.26
-
 D-arabitol pH 10.0, 40°C Aeribacillus pallidus
0.87
-
 L-xylulose pH 10.0, 40°C Aeribacillus pallidus
1.33
-
 xylitol pH 10.0, 40°C Aeribacillus pallidus
3.31
-
 D-threitol pH 10.0, 40°C Aeribacillus pallidus