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Literature summary for 1.1.1.B19 extracted from

  • Li, M.; Zhu, W.; Meng, Q.; Miao, M.; Zhang, T.
    Characterization of xylitol 4-dehydrogenase from Erwinia aphidicola and its co-expression with NADH oxidase in Bacillus subtilis (2021), Process Biochem., 104, 92-100 .
No PubMed abstract available
Search for more literature for 1.1.1.B19

Application

Application Comment Organism
synthesis enzyme XDH from Erwinia aphidicola can be useful for production of L-xylulose, a rare ketose, for apllication in pharmaceutical and food industries Erwinia aphidicola

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Bacillus subtilis, coexpression with the water-forming enzyme NADH oxidase (NOX) for NAD+ recycling leading to 3.4fold increased XDH activity Erwinia aphidicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18.71
-
 xylitol recombinant enzyme, pH 11.0, 45°C Erwinia aphidicola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Erwinia aphidicola
-
 L-xylulose + NADH + H+
-
 ?
xylitol + NAD+ Erwinia aphidicola SK47.001
-
 L-xylulose + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Erwinia aphidicola
-
-
-
Erwinia aphidicola SK47.001
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Bacillus subtilis Erwinia aphidicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
 Erwinia aphidicola L-xylulose + NADH + H+
-
 ?
xylitol + NAD+
-
 Erwinia aphidicola SK47.001 L-xylulose + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
XDH
-
 Erwinia aphidicola
xylitol 4-dehydrogenase
-
 Erwinia aphidicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant enzyme Erwinia aphidicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
 recombinant enzyme Erwinia aphidicola

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Erwinia aphidicola

General Information

General Information Comment Organism
additional information enzyme structure homology modelling and molecular docking, active site structure analysis, the potential catalytic sites are Ser149, Tyr162, and Lys166 for xylitol and NAD+ Erwinia aphidicola