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Literature summary for 1.1.1.B3 extracted from

  • You, Z.Y.; Liu, Z.Q.; Zheng, Y.G.
    Characterization of a newly synthesized carbonyl reductase and construction of a biocatalytic process for the synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate with high space-time yield (2014), Appl. Microbiol. Biotechnol., 98, 1671-1680.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not inhibitory or activating: EDTA, dithiothreitol Candida parapsilosis
Triton X-100 1%, 112% of initial activity Candida parapsilosis
Tween-20 1%, 111% of initial activity Candida parapsilosis
Tween-80 1%, 115% of initial activity Candida parapsilosis

Application

Application Comment Organism
synthesis using recombinant Scr2 in an aqueous-organic solvent system with a substrate fed-batch strategy and a final substrate concentration of 1 M, a yield of 95.3% and e.e. of 99% is obtained after 6-h reaction Candida parapsilosis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, no residual activity Candida parapsilosis
Cu2+ 1 mM, no residual activity Candida parapsilosis
Fe2+ 1 mM, 2.5% residual activity Candida parapsilosis
Fe3+ 1 mM, 44% residual activity Candida parapsilosis
Mg2+ 1 mM, 77% residual activity Candida parapsilosis
additional information not inhibitory or activating: EDTA, dithiothreitol Candida parapsilosis
SDS 1%, no residual activity Candida parapsilosis
Zn2+ 1 mM, 79% residual activity Candida parapsilosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4
-
4-chloro-3-oxobutanoate pH 6.0, 45°C Candida parapsilosis

Organic Solvent Stability

Organic Solvent Comment Organism
benzene 85% residual activity Candida parapsilosis
cyclohexane 88% residual activity Candida parapsilosis
dimethyl formamide 5%, 82% residual activity Candida parapsilosis
dimethyl phthallate 131% residual activity Candida parapsilosis
dimethyl sulfoxide 5%, 89% residual activity Candida parapsilosis
heptane 101% residual activity Candida parapsilosis
hexane 74% residual activity Candida parapsilosis
isooctane 106% residual activity Candida parapsilosis

Organism

Organism UniProt Comment Textmining
Candida parapsilosis D5G304
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butanedione + NADPH + H+
-
Candida parapsilosis ? + NADP+
-
?
4-chloro-3-oxobutanoate + NADH + H+
-
Candida parapsilosis (S)-4-chloro-3-hydroxybutanoate + NAD+ 99% enantiomeric excess of (S)-enantiomer ?
ethyl 3-oxobutanoate + NADPH + H+
-
Candida parapsilosis ethyl (S)-3-hydroxybutanoate + NADP+
-
?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+
-
Candida parapsilosis ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
?
methyl 4-chloro-3-oxobutanoate + NADPH + H+
-
Candida parapsilosis methyl (S)-4-chloro-3-hydroxybutanoate + NADP+
-
?
additional information no substrates: cetophenone, 2-hydroxyacetophenone, acetone, butanone, 2-pentanone, 3-pentanone, 2-hexanone, 2-heptanone, 2-octanone, cyclohexanone, acetylacetone, 2,5-hexanedione and 5,5-dimethyl-l,3-cyclohexanedione Candida parapsilosis ?
-
?

Synonyms

Synonyms Comment Organism
Scr2
-
Candida parapsilosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Candida parapsilosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
half-life 16.5 h Candida parapsilosis
40
-
half-life 2.2 h Candida parapsilosis
50
-
half-life 0.05 h Candida parapsilosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Candida parapsilosis