Literature summary for 1.1.1.B3 extracted from

Cai, P.; An, M.; Xu, S.; Yan, M.; Hao, N.; Li, Y.; Xu, L.
Asymmetric synthesis of (S)-4-chloro-3-hydroxybutanoate by sorbose reductase from Candida albicans with two co-existing recombinant Escherichia coli strains (2015), Biosci. Biotechnol. Biochem., 79, 1090-1093.

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Application

Application	Comment	Organism
synthesis	the enzyme catalyzes the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, the activity is 6.2 U/mg. Using two coexisting recombinant Escherichia coli strains, in which a strain expressing glucose dehydrogenase is used as an NADPH regenerator. An optical purity of 99% (e.e.) and a maximum yield of 1240 mM (S)-4-chloro-3-hydroxybutanoate are obtained, and highest turnover number of 53900 can be achieved without adding extra NADP+/NADPH	Candida albicans

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	P87219	cf. EC 1.1.1.289	-
Candida albicans ATCC MYA-2876	P87219	cf. EC 1.1.1.289	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ethyl 4-chloro-3-oxobutanoate + NADPH + H+	-	Candida albicans	ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+	the enzyme catalyzes the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, 99% enantiomeric excess	?
ethyl 4-chloro-3-oxobutanoate + NADPH + H+	-	Candida albicans ATCC MYA-2876	ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+	the enzyme catalyzes the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, 99% enantiomeric excess	?

Synonyms

Synonyms	Comment	Organism
SOU1	-	Candida albicans

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Release 2023.1 (January 2023)