Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
enzyme bound to 3-hydroxy-3-methylglutaryl-CoA, hanging drop vapor diffusion method, using 100 mM Tris pH 8.5, 100-250 mM lithium sulfate, and 15-25% (w/v) PEG 4000. Enzyme bound to NADPH, hanging drop vapor diffusion method, using 100 mM Tris pH 8.5, 100-250 mM lithium sulfate, and 30-40% (w/v) PEG 4000 | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0289 | - |
NADPH | at pH 7.4 and 37°C | Streptococcus pneumoniae | |
0.153 | - |
NADH | at pH 7.4 and 37°C | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | Streptococcus pneumoniae | - |
mevalonate + CoA + 2 NAD+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | Streptococcus pneumoniae | - |
mevalonate + CoA + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
Streptococcus pneumoniae | mevalonate + CoA + 2 NAD+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
Streptococcus pneumoniae | mevalonate + CoA + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl coenzyme A reductase | - |
Streptococcus pneumoniae |
class II HMG-CoA reductase | - |
Streptococcus pneumoniae |
HGMR | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.131 | - |
NADH | at pH 7.4 and 37°C | Streptococcus pneumoniae | |
6.85 | - |
NADPH | at pH 7.4 and 37°C | Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Streptococcus pneumoniae | |
NADPH | kinetically preferred cofactor | Streptococcus pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in the mevalonate pathway | Streptococcus pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.86 | - |
NADH | at pH 7.4 and 37°C | Streptococcus pneumoniae | |
240 | - |
NADPH | at pH 7.4 and 37°C | Streptococcus pneumoniae |