BRENDA - Enzyme Database show
show all sequences of 1.1.1.B60

A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization

Kim, T.S.; Patel, S.K.; Selvaraj, C.; Jung, W.S.; Pan, C.H.; Kang, Y.C.; Lee, J.K.; Sci. Rep. 6, 33438 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)-CodonPlus RIL cells
Gluconobacter oxydans
General Stability
General Stability
Organism
stability of the enzyme significantly improves (up to 13.6fold) after cross-linking of immobilized enzyme on silica nanoparticles and retains 62.8% residual activity after 10 cycles of reuse
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-sorbitol
inhibitory at more than 10% (w/v)
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
38.9
-
D-sorbitol
at pH 10.0 and 25C
Gluconobacter oxydans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
activates
Gluconobacter oxydans
Ca2+
activates
Gluconobacter oxydans
Cu2+
activates
Gluconobacter oxydans
K+
activates
Gluconobacter oxydans
additional information
Mg2+, Co2+, and Mn2+ ions show no stimulatory influence on enzyme activity
Gluconobacter oxydans
Zn2+
activates
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
2 * 54000, SDS-PAGE
Gluconobacter oxydans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
-
-
Gluconobacter oxydans G624
-
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NADP+
-
741426
Gluconobacter oxydans
?
-
-
-
?
D-sorbitol + NADP+
best substrate
741426
Gluconobacter oxydans
L-sorbose + NADPH + H+
-
-
-
?
D-sorbitol + NADP+
best substrate
741426
Gluconobacter oxydans G624
L-sorbose + NADPH + H+
-
-
-
?
mannitol + NADP+
-
741426
Gluconobacter oxydans
?
-
-
-
?
additional information
no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
741426
Gluconobacter oxydans
?
-
-
-
-
additional information
no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
741426
Gluconobacter oxydans G624
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 54000, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Gluconobacter oxydans
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
75
more than 40% activity between 25 and 75C
Gluconobacter oxydans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3820
-
D-sorbitol
at pH 10.0 and 25C
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
the enzyme exhibits high preference for NADP+ (vs. 2.5% relative activity with NAD+)
Gluconobacter oxydans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3)-CodonPlus RIL cells
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
the enzyme exhibits high preference for NADP+ (vs. 2.5% relative activity with NAD+)
Gluconobacter oxydans
General Stability (protein specific)
General Stability
Organism
stability of the enzyme significantly improves (up to 13.6fold) after cross-linking of immobilized enzyme on silica nanoparticles and retains 62.8% residual activity after 10 cycles of reuse
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-sorbitol
inhibitory at more than 10% (w/v)
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
38.9
-
D-sorbitol
at pH 10.0 and 25C
Gluconobacter oxydans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
activates
Gluconobacter oxydans
Ca2+
activates
Gluconobacter oxydans
Cu2+
activates
Gluconobacter oxydans
K+
activates
Gluconobacter oxydans
additional information
Mg2+, Co2+, and Mn2+ ions show no stimulatory influence on enzyme activity
Gluconobacter oxydans
Zn2+
activates
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
2 * 54000, SDS-PAGE
Gluconobacter oxydans
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-arabinitol + NADP+
-
741426
Gluconobacter oxydans
?
-
-
-
?
D-sorbitol + NADP+
best substrate
741426
Gluconobacter oxydans
L-sorbose + NADPH + H+
-
-
-
?
D-sorbitol + NADP+
best substrate
741426
Gluconobacter oxydans G624
L-sorbose + NADPH + H+
-
-
-
?
mannitol + NADP+
-
741426
Gluconobacter oxydans
?
-
-
-
?
additional information
no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
741426
Gluconobacter oxydans
?
-
-
-
-
additional information
no activity is detected with L-arabinitol, xylitol, ribitol, myo-inositol, and glycerol
741426
Gluconobacter oxydans G624
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 54000, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Gluconobacter oxydans
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
75
more than 40% activity between 25 and 75C
Gluconobacter oxydans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3820
-
D-sorbitol
at pH 10.0 and 25C
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Gluconobacter oxydans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
98.1
-
D-sorbitol
at pH 10.0 and 25C
Gluconobacter oxydans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
98.1
-
D-sorbitol
at pH 10.0 and 25C
Gluconobacter oxydans
Other publictions for EC 1.1.1.B60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741426
Kim
A highly efficient sorbitol de ...
Gluconobacter oxydans, Gluconobacter oxydans G624
Sci. Rep.
6
33438
2016
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