Literature summary for 1.1.1.B60 extracted from

Kim, T.S.; Gao, H.; Li, J.; Kalia, V.C.; Muthusamy, K.; Sohng, J.K.; Kim, I.W.; Lee, J.K.
Overcoming NADPH product inhibition improves D-sorbitol conversion to L-sorbose (2019), Sci. Rep., 9, 815 .

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Application

Application	Comment	Organism
synthesis	synthesis of L-sorbose is largely used as a starting material for L-ascorbic acid biosynthesis. It can been also used to synthesize the potent glycosidase inhibitor 1-deoxygalactonojirim and rare sugars such as L-tagatose and L-iditol. Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol	Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol	Gluconobacter oxydans

Cloned (Comment)

Organism

Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol

Gluconobacter oxydans

Inhibitors

Inhibitors	Comment	Organism	Structure
NADPH	product inhibition. Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol	Gluconobacter oxydans

Organism

Organism	UniProt	Comment	Textmining
Gluconobacter oxydans	-	-	-
Gluconobacter oxydans G624	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-sorbitol + NADP+	strict NADP+-dependent	Gluconobacter oxydans	L-sorbose + NADPH + H+	-	?
D-sorbitol + NADP+	strict NADP+-dependent	Gluconobacter oxydans G624	L-sorbose + NADPH + H+	-	?

Synonyms

Synonyms	Comment	Organism
GoSLDH	-	Gluconobacter oxydans
sorbitol dehydrogenase	-	Gluconobacter oxydans