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Literature summary for 1.1.2.10 extracted from

  • Good, N.M.; Vu, H.N.; Suriano, C.J.; Subuyuj, G.A.; Skovran, E.; Martinez-Gomez, N.C.
    Pyrroloquinoline quinone ethanol dehydrogenase in Methylobacterium extorquens AM1 extends lanthanide-dependent metabolism to multicarbon substrates (2016), J. Bacteriol., 198, 3109-3118 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Methylorubrum extorquens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
ethanol at pH 9.0 and 30┬░C Methylorubrum extorquens
0.066
-
formaldehyde at pH 9.0 and 30┬░C Methylorubrum extorquens
0.285
-
acetaldehyde at pH 9.0 and 30┬░C Methylorubrum extorquens
5.98
-
methanol at pH 9.0 and 30┬░C Methylorubrum extorquens

Metals/Ions

Metals/Ions Comment Organism Structure
La3+ the enzyme preferentially binds La3+ over Ca2+ in the active site. 0.1 mM used in assay conditions. The enzyme contains 1.3 mol of La3+ per mol of protomer Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
126400
-
SEC-MALS Methylorubrum extorquens

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens C5AXV8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + phenazine methosulfate highest activity Methylorubrum extorquens acetaldehyde + reduced phenazine methosulfate
-
r
formaldehyde + reduced phenazine methosulfate the enzyme is 100times more efficient at oxidizing formaldehyde than methanol Methylorubrum extorquens methanol + phenazine methosulfate
-
r
methanol + phenazine methosulfate
-
Methylorubrum extorquens formaldehyde + reduced phenazine methosulfate
-
r

Subunits

Subunits Comment Organism
homodimer x * 61000, SDS-PAGE Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
exaF
-
Methylorubrum extorquens
pyrroloquinoline quinone ethanol dehydrogenase
-
Methylorubrum extorquens
quinoprotein ethanol dehydrogenase
-
Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
Methylorubrum extorquens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2
-
methanol at pH 9.0 and 30┬░C Methylorubrum extorquens
20
-
acetaldehyde at pH 9.0 and 30┬░C Methylorubrum extorquens
259
-
formaldehyde at pH 9.0 and 30┬░C Methylorubrum extorquens
14500
-
ethanol at pH 9.0 and 30┬░C Methylorubrum extorquens