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Literature summary for 1.1.2.10 extracted from

  • Lumpe, H.; Pol, A.; Op den Camp, H.J.M.; Daumann, L.J.
    Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase - a kinetic and DFT study (2018), Dalton Trans., 47, 10463-10472 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
methanol in the presence of a mixture of La3+, Ce3+, Pr3+, Nd3+, at pH 7.2 and 60┬░C Methylacidiphilum fumariolicum
0.00082
-
methanol in the presence of 0.02 mM Eu3+ and Lu3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
0.00091
-
methanol in the presence of 0.02 mM Eu3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
0.00132
-
methanol in the presence of 0.02 mM Eu3+ and La3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
0.00362
-
methanol in the presence of 0.02 mM Eu3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum

Metals/Ions

Metals/Ions Comment Organism Structure
Eu3+ the addition of increasing amounts of europium(III) to 200 nM purified partial-apo enzyme leads to a gradual increase in activity until saturation around 0.005 to 0.02 mM added metal is observed Methylacidiphilum fumariolicum
La3+ between 0 and 0.005 mM La3+ a sharp increase in enzyme activity is observed Methylacidiphilum fumariolicum
Nd3+ between 0 and 0.005 mM La3+ a sharp increase in enzyme activity is observed Methylacidiphilum fumariolicum
Pr3+ between 0 and 0.005 mM La3+ a sharp increase in enzyme activity is observed Methylacidiphilum fumariolicum

Organism

Organism UniProt Comment Textmining
Methylacidiphilum fumariolicum
-
-
-
Methylacidiphilum fumariolicum SolV
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + phenazine ethosulfate
-
Methylacidiphilum fumariolicum formaldehyde + reduced phenazine ethosulfate
-
?
methanol + phenazine ethosulfate
-
Methylacidiphilum fumariolicum SolV formaldehyde + reduced phenazine ethosulfate
-
?

Synonyms

Synonyms Comment Organism
Eu-MDH
-
Methylacidiphilum fumariolicum
Ln-dependent MDH
-
Methylacidiphilum fumariolicum
Ln-MDH
-
Methylacidiphilum fumariolicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26
-
methanol in the presence of 0.02 mM Eu3+ and Lu3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
50
-
methanol in the presence of 0.02 mM Eu3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
55
-
methanol in the presence of 0.02 mM Eu3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
123
-
methanol in the presence of 0.02 mM Eu3+ and La3+, at pH 7.2 and 45┬░C Methylacidiphilum fumariolicum
5800
-
methanol in the presence of a mixture of La3+, Ce3+, Pr3+, Nd3+, at pH 7.2 and 60┬░C Methylacidiphilum fumariolicum