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Literature summary for 1.1.2.10 extracted from

  • Huang, J.; Yu, Z.; Chistoserdova, L.
    Lanthanide-dependent methanol dehydrogenases of XoxF4 and XoxF5 clades are differentially distributed among methylotrophic bacteria and they reveal different biochemical properties (2018), Front. Microbiol., 9, 1366 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
ammonia ammonia reveals a slight inhibitory effect on subtype XoxF4-2 Methylotenera mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
1-Hexanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
0.04
-
1-Hexanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
0.042
-
methanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
0.055
-
methanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
0.059
-
ethanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
0.061
-
1-propanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
0.085
-
ethanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
0.096
-
1-butanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
0.133
-
1-propanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
0.188
-
1-butanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
0.357
-
1-Hexanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
0.432
-
formaldehyde subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
0.464
-
formaldehyde at pH 9.0 and 26┬░C Methylomonas sp. LW13
0.47
-
1-butanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
0.515
-
ethanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
0.815
-
formaldehyde subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
0.873
-
1-propanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ce3+ activates Methylotenera mobilis
Ce3+ activates Methylomonas sp. LW13
Gd3+ activates Methylotenera mobilis
La3+ activates Methylotenera mobilis
La3+ activates Methylomonas sp. LW13
additional information subtype XoxF4-1 can use lighter lanthanides up to the atomic number of 64 (La3+ through Gd3+) while subtype XoxF4-2 can only use lanthanides up to the atomic number of 62 (La3+ through Sm3+) Methylotenera mobilis
additional information the enzyme has a requirement for ammonia Methylomonas sp. LW13
Nd3+ activates Methylotenera mobilis
Nd3+ activates Methylomonas sp. LW13
Sm3+ activates Methylotenera mobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
127900
-
calculated from amino acid sequence Methylomonas sp. LW13
129400
-
subtype XoxF4-1, calculated from amino acid sequence Methylotenera mobilis
131700
-
subtype XoxF4-2, calculated from amino acid sequence Methylotenera mobilis

Organism

Organism UniProt Comment Textmining
Methylomonas sp. LW13
-
-
-
Methylotenera mobilis
-
-
-
Methylotenera mobilis JLW8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Methylotenera mobilis
Ni-NTA agarose column chromatography Methylomonas sp. LW13

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:methanol-grown cell
-
Methylotenera mobilis
-
culture condition:methanol-grown cell
-
Methylomonas sp. LW13
-

Storage Stability

Storage Stability Organism
0-25┬░C, the enzyme loses approximately 50% of its activity within 24 h whether maintained, on ice, in pH 8.0, pH 8.5, or pH 9.0 Tris buffer supplemented with dithiothreitol and glycerol Methylotenera mobilis
0-25┬░C, the enzyme loses approximately 50% of its activity within 24 h whether maintained, on ice, in pH 8.0, pH 8.5, or pH 9.0 Tris buffer supplemented with dithiothreitol and glycerol Methylomonas sp. LW13

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis butyraldehyde + reduced phenazine methosulfate
-
r
1-butanol + phenazine methosulfate more than 95% activity compared to methanol Methylomonas sp. LW13 butyraldehyde + reduced phenazine methosulfate
-
r
1-hexanol + phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis hexaldehyde + reduced phenazine methosulfate
-
r
1-hexanol + phenazine methosulfate more than 95% activity compared to methanol Methylomonas sp. LW13 hexaldehyde + reduced phenazine methosulfate
-
r
1-propanol + phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis propionaldehyde + reduced phenazine methosulfate
-
r
1-propanol + phenazine methosulfate more than 95% activity compared to methanol Methylomonas sp. LW13 propionaldehyde + reduced phenazine methosulfate
-
r
1-propanol + phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis JLW8 propionaldehyde + reduced phenazine methosulfate
-
r
2-propanol + phenazine methosulfate 40% activity compared to methanol Methylomonas sp. LW13 ?
-
r
2-propanol + phenazine methosulfate subtype XoxF4-1 shows 20% activity compared to methanol, subtype XoxF4-2 shows 5% activity compared to methanol Methylotenera mobilis ?
-
r
acetaldehyde + reduced phenazine methosulfate 60% activity compared to methanol Methylomonas sp. LW13 ethanol + phenazine methosulfate
-
r
acetaldehyde + reduced phenazine methosulfate subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol Methylotenera mobilis ethanol + phenazine methosulfate
-
r
acetaldehyde + reduced phenazine methosulfate subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol Methylotenera mobilis JLW8 ethanol + phenazine methosulfate
-
r
ethanol + phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis acetaldehyde + reduced phenazine methosulfate
-
r
ethanol + phenazine methosulfate more than 95% activity compared to methanol Methylomonas sp. LW13 acetaldehyde + reduced phenazine methosulfate
-
r
ethanol + phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis JLW8 acetaldehyde + reduced phenazine methosulfate
-
r
formaldehyde + reduced phenazine methosulfate more than 95% activity compared to methanol Methylotenera mobilis methanol + phenazine methosulfate
-
r
formaldehyde + reduced phenazine methosulfate more than 95% activity compared to methanol Methylomonas sp. LW13 methanol + phenazine methosulfate
-
r
methanol + phenazine methosulfate 100% activity Methylotenera mobilis formaldehyde + reduced phenazine methosulfate
-
r
methanol + phenazine methosulfate 100% activity Methylomonas sp. LW13 formaldehyde + reduced phenazine methosulfate
-
r
methanol + phenazine methosulfate 100% activity Methylotenera mobilis JLW8 formaldehyde + reduced phenazine methosulfate
-
r
propionaldehyde + reduced phenazine methosulfate 60% activity compared to methanol Methylomonas sp. LW13 1-propanol + phenazine methosulfate
-
r
propionaldehyde + reduced phenazine methosulfate subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol Methylotenera mobilis 1-propanol + phenazine methosulfate
-
r
propionaldehyde + reduced phenazine methosulfate subtype XoxF4-1 shows 50% activity compared to methanol, subtype XoxF4-2 shows 15% activity compared to methanol Methylotenera mobilis JLW8 1-propanol + phenazine methosulfate
-
r

Subunits

Subunits Comment Organism
homodimer
-
Methylotenera mobilis
homodimer
-
Methylomonas sp. LW13

Synonyms

Synonyms Comment Organism
lanthanide-dependent alcohol dehydrogenase
-
Methylotenera mobilis
lanthanide-dependent alcohol dehydrogenase
-
Methylomonas sp. LW13
XoxF4-1 subtype, dominant type in nature Methylotenera mobilis
XoxF4-2 subtype Methylotenera mobilis
XoxF5
-
Methylomonas sp. LW13

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
subtype XoxF4-2 Methylotenera mobilis
9
-
-
Methylomonas sp. LW13
9
-
subtype XoxF4-1 Methylotenera mobilis

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 the enzyme shows more than 50% activity between pH 7.5 and 9.5 Methylotenera mobilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.73
-
1-propanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
2.15
-
1-butanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
2.48
-
formaldehyde subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
3.97
-
formaldehyde subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
5.54
-
1-Hexanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
5.99
-
ethanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
16.63
-
1-butanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
27.03
-
ethanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
27.17
-
methanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
34.88
-
1-Hexanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
35.36
-
1-propanol subtype XoxF4-1, at pH 9.0 and 26┬░C Methylotenera mobilis
37.86
-
formaldehyde at pH 9.0 and 26┬░C Methylomonas sp. LW13
42.19
-
methanol subtype XoxF4-2, at pH 9.0 and 26┬░C Methylotenera mobilis
112.54
-
1-propanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
133.9
-
1-butanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
159.15
-
ethanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
342.55
-
methanol at pH 9.0 and 26┬░C Methylomonas sp. LW13
415.52
-
1-Hexanol at pH 9.0 and 26┬░C Methylomonas sp. LW13