Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.3 extracted from

  • Smutok, O.; Dmytruk, K.; Gonchar, M.; Sibirny, A.; Schuhmann, W.
    Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors (2007), Biosens. Bioelectron., 23, 599-605.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
analysis a L-lactate-selective microbial biosensor is developed using permeabilized cells of gene-engineered thermotolerant methylotrophic yeast Hansenula polymorpha, over-producing FCb2. The HpCYB2 gene, encoding FCb2, under the control of the strong Hansenula polymorpha alcohol oxidase promoter in the frame of a plasmid for multicopy integration is transformed to the recipient strain Hansenula polymorpha C-105 (gcr1 catX) impaired in glucose repression and devoid of catalase activity. The biosensor based on recombinant yeast cells exhibit a higher Km value (Km: 3.02 mM) and hence expanded linear range toward l-lactate as compared to a similar sensor based on the initial cells of Hansenula polymorpha C-105 (Km: 0.33 mM) Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
L-lactate sensor based on the inital Hansenula polymorpha C-105 cells Ogataea angusta
3.02
-
L-lactate biosensor based on recombinant yeast FCb2cells Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + potassium ferricyanide
-
Ogataea angusta pyruvate + potassium ferrocyanide + H+
-
?

Synonyms

Synonyms Comment Organism
Fcb2
-
Ogataea angusta
flavocytochrome b2
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Ogataea angusta