Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.3 extracted from

  • Mowat, C.G.; Gazur, B.; Campbell, L.P.; Chapman, S.K.
    Flavin-containing heme enzymes (2010), Arch. Biochem. Biophys., 493, 37-52.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H373Q the mutation results in a 3–4 orders of magnitude decrease in kcat and a slight increase in L-lactate Km Saccharomyces cerevisiae
L230A the mutant flavocytochrome b2 displays increased selectivity for (S)-2-hydroxyoctanoate over L-lactate by a factor of 40 (kcat/Km) Saccharomyces cerevisiae
L230A/A198G the double mutant enzyme has a 6fold greater catalytic efficiency with L-mandelate than with L-lactate Saccharomyces cerevisiae
Y254F the mutant enzyme has a Vmax value some 28fold lower than that of the wild type enzyme and a slightly raised L-lactate Km value Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
4 * 57500, X-ray crystallography Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + ferricytochrome c Saccharomyces cerevisiae
-
pyruvate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00175
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 57500, X-ray crystallography Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
flavocytochrome b2
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Saccharomyces cerevisiae
heme
-
Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae flavocytochrome b2 production is induced by the presence of oxygen and L-lactate up

General Information

General Information Comment Organism
metabolism the pyruvate produced as a result of L-lactate oxidation is utilised by the Krebs cycle, but flavocytochrome b2 also forms part of a short respiratory electron transport chain which results in one ATP molecule being produced for every L-lactate consumed Saccharomyces cerevisiae