Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.3 extracted from

  • Le, K.H.D.; Boussac, A.; Frangioni, B.; Leger, C.; Lederer, F.
    Interdomain contacts in flavocytochrome b2, a mutational analysis (2009), Biochemistry, 48, 10803-10809.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A67L site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
A67Q site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
D72A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
E63K site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
F39A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
K73A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
L65A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
additional information introduction of a number of heme domain side chain substitutions in and around the interface to probe their effect on flavin to heme and cytochrome b2 electron transfer, overview Saccharomyces cerevisiae
N69K site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
P44A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
P64Q site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
P64R site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
V70M site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
Y74F site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information stopped-flow, pre-steady-state and steady-state kinetics measuring electron transfer in artificial systems, redox potentials, overview Saccharomyces cerevisiae
0.02
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A Saccharomyces cerevisiae
0.032
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q Saccharomyces cerevisiae
0.051
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A Saccharomyces cerevisiae
0.061
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R Saccharomyces cerevisiae
0.065
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
0.069
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q Saccharomyces cerevisiae
0.085
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L Saccharomyces cerevisiae
0.092
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K Saccharomyces cerevisiae
0.097
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A Saccharomyces cerevisiae
0.105
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M Saccharomyces cerevisiae
0.109
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A Saccharomyces cerevisiae
0.113
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
0.119
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K Saccharomyces cerevisiae
0.131
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion intermembrane space Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme cofactor Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + ferricytochrome c Saccharomyces cerevisiae flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source pyruvate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00175
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + cytochrome c
-
Saccharomyces cerevisiae pyruvate + oxidized cytochrome c
-
?
L-lactate + ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
?
L-lactate + ferricytochrome c flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
?
additional information during the catalytic cycle, electrons are transferred one by one from the reduced flavin to heme b2 in the same subunit Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
tetramer
-
Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
flavocytochrome b2
-
Saccharomyces cerevisiae
L-lactate cytochrome c oxidoreductase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q Saccharomyces cerevisiae
39
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
62
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
63
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A Saccharomyces cerevisiae
87
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A Saccharomyces cerevisiae
108
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L Saccharomyces cerevisiae
139
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K Saccharomyces cerevisiae
143
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R Saccharomyces cerevisiae
155
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme Saccharomyces cerevisiae
164
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A Saccharomyces cerevisiae
165
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A Saccharomyces cerevisiae
168
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q Saccharomyces cerevisiae
171
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M Saccharomyces cerevisiae
184
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K Saccharomyces cerevisiae
185
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q Saccharomyces cerevisiae
190
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A Saccharomyces cerevisiae
196
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
207
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q Saccharomyces cerevisiae
208
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L Saccharomyces cerevisiae
209
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
211
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A Saccharomyces cerevisiae
212
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R Saccharomyces cerevisiae
214
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A Saccharomyces cerevisiae
214
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme Saccharomyces cerevisiae
218
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K Saccharomyces cerevisiae
218
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K Saccharomyces cerevisiae
223
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A Saccharomyces cerevisiae
225
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
cytochrome c the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain Saccharomyces cerevisiae
FAD the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain Saccharomyces cerevisiae
heme the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c and enables the latter to grow on lactate as the sole carbon source Saccharomyces cerevisiae