Protein Variants | Comment | Organism |
---|---|---|
A67L | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
A67Q | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
D72A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
E63K | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
F39A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
K73A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
L65A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
additional information | introduction of a number of heme domain side chain substitutions in and around the interface to probe their effect on flavin to heme and cytochrome b2 electron transfer, overview | Saccharomyces cerevisiae |
N69K | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
P44A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
P64Q | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
P64R | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
V70M | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
Y74F | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow, pre-steady-state and steady-state kinetics measuring electron transfer in artificial systems, redox potentials, overview | Saccharomyces cerevisiae | |
0.02 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A | Saccharomyces cerevisiae | |
0.032 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q | Saccharomyces cerevisiae | |
0.051 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A | Saccharomyces cerevisiae | |
0.061 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R | Saccharomyces cerevisiae | |
0.065 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
0.069 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q | Saccharomyces cerevisiae | |
0.085 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L | Saccharomyces cerevisiae | |
0.092 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K | Saccharomyces cerevisiae | |
0.097 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A | Saccharomyces cerevisiae | |
0.105 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M | Saccharomyces cerevisiae | |
0.109 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A | Saccharomyces cerevisiae | |
0.113 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
0.119 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K | Saccharomyces cerevisiae | |
0.131 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | intermembrane space | Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme cofactor | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + ferricytochrome c | Saccharomyces cerevisiae | flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source | pyruvate + ferrocytochrome c + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P00175 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + cytochrome c | - |
Saccharomyces cerevisiae | pyruvate + oxidized cytochrome c | - |
? | |
L-lactate + ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c + H+ | - |
? | |
L-lactate + ferricytochrome c | flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source | Saccharomyces cerevisiae | pyruvate + ferrocytochrome c + H+ | - |
? | |
additional information | during the catalytic cycle, electrons are transferred one by one from the reduced flavin to heme b2 in the same subunit | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
flavocytochrome b2 | - |
Saccharomyces cerevisiae |
L-lactate cytochrome c oxidoreductase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q | Saccharomyces cerevisiae | |
39 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
62 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
63 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A | Saccharomyces cerevisiae | |
87 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A | Saccharomyces cerevisiae | |
108 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L | Saccharomyces cerevisiae | |
139 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K | Saccharomyces cerevisiae | |
143 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R | Saccharomyces cerevisiae | |
155 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme | Saccharomyces cerevisiae | |
164 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A | Saccharomyces cerevisiae | |
165 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A | Saccharomyces cerevisiae | |
168 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q | Saccharomyces cerevisiae | |
171 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M | Saccharomyces cerevisiae | |
184 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K | Saccharomyces cerevisiae | |
185 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q | Saccharomyces cerevisiae | |
190 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A | Saccharomyces cerevisiae | |
196 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
207 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q | Saccharomyces cerevisiae | |
208 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L | Saccharomyces cerevisiae | |
209 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
211 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A | Saccharomyces cerevisiae | |
212 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R | Saccharomyces cerevisiae | |
214 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A | Saccharomyces cerevisiae | |
214 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme | Saccharomyces cerevisiae | |
218 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K | Saccharomyces cerevisiae | |
218 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K | Saccharomyces cerevisiae | |
223 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A | Saccharomyces cerevisiae | |
225 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain | Saccharomyces cerevisiae | |
FAD | the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain | Saccharomyces cerevisiae | |
heme | the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c and enables the latter to grow on lactate as the sole carbon source | Saccharomyces cerevisiae |