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Literature summary for 1.1.2.3 extracted from

  • Karkovska, M.; Smutok, O.; Stasyuk, N.; Gonchar, M.
    L-Lactate-selective microbial sensor based on flavocytochrome b2-enriched yeast cells using recombinant and nanotechnology approaches (2015), Talanta, 144, 1195-1200 .
    View publication on PubMed

Application

Application Comment Organism
analysis lactate-selective microbial sensor based on flavocytochrome b2-enriched yeast cells Ogataea angusta

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of the enzyme in Hansenula polymorpha yeast cells additionally enriched by recombinant enzyme bound with gold nanoparticles , FC b2-nAu Ogataea angusta

Protein Variants

Protein Variants Comment Organism
additional information construction of a lactate-selective microbial sensor based on flavocytochrome b2-enriched yeast cells. Combination of recombinant technology and nanotechnology by recombinant overexpression of the enzyme in Hansenula polymorpha yeast cells additionally enriched by recombinant enzyme bound with gold nanoparticles, FC b2-nAu. The FC b2-nAu-enriched livingand permeabilized yeast cells are used for construction of a bioselective membrane of microbial L-lactate-selective amperometric biosensor. Phenazine methosulfate serves as a free defusing electron transfer mediator which provides effective electron transfer from the reduced enzyme to the electrode surface. The output to L-lactate of FC b2-nAu-enriched permeabilized yeast cells is 2.5fold higher compared to control cells. For intact cells, the treatment with FC b2-nAu results in 1.56fold increased enzymatic activity. For permeabilized cells, the enrichment efficacy is 2.33fold increased. Method evaluation Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta W1QKE8
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
recombinant overexpressed enzyme in crude cell-free extract, pH 7.8, 30°C Ogataea angusta
4.45
-
recombinant overexpressed enzyme in permeabilized cells, pH 7.8, 30°C Ogataea angusta

Subunits

Subunits Comment Organism
homotetramer the enzyme is a tetramer with four identical subunits, each consisting of FMN- and heme-binding domains Ogataea angusta

Synonyms

Synonyms Comment Organism
FC b2
-
Ogataea angusta
flavocytochrome b2
-
Ogataea angusta
L-lactate-cytochrome c-oxidoreductase
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Ogataea angusta